Rabbit anti-Human CDCA8 Polyclonal Antibody | anti-CDCA8 antibody

CDCA8, ID (CDCA8, PESCRG3, Borealin, Dasra-B, Cell division cycle-associated protein 8, Pluripotent embryonic stem cell-related gene 3 protein) (Azide free) (HRP)

Cat. Num. AAA6284032

• Gene Names: CDCA8; BOR; DasraB; MESRGP; BOREALIN
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDCA8; Polyclonal Antibody; ID (CDCA8; PESCRG3; Borealin; Dasra-B; Cell division cycle-associated protein 8; Pluripotent embryonic stem cell-related gene 3 protein) (Azide free) (HRP); Pluripotent embryonic stem cell-related gene 3 protein; anti-CDCA8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CDCA8 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CDCA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~152 amino acids from the center region of human CDCA8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDCA8 Antibody (Center) in HL60 cell line lysates (35ug/lane). CDCA8(arrow) was detected using the purified polyclonal antibody.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HL-60 cells using CDCA8 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDCA8 antibody

CDCA8 is a component of the chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The CPC complex, a key regulator of mitosis, has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 may be required to direct the CPC to centromeric DNA.

Product Categories/Family for anti-CDCA8 antibody

Antibodies; Cell Division Cycle (CDC)

References

Sampath, S.C., et al., Cell 118(2):187-202 (2004). Gassmann, R., et al., J. Cell Biol. 166(2):179-191 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 110832774
• NCBI GeneID: 55143
• UniProt Accession #: Q53HL2; Q53HN1; Q96AM3; Q9NVW5; D3DPT4
• Molecular Weight: 280
• NCBI Official Full Name: Borealin
• NCBI Official Synonym Full Names: cell division cycle associated 8
• NCBI Official Symbol: CDCA8
• NCBI Official Synonym Symbols: BOR; DasraB; MESRGP; BOREALIN
• NCBI Protein Information: borealin; Dasra B; dasra-B; hDasra-B; cell division cycle-associated protein 8; pluripotent embryonic stem cell-related gene 3 protein
• UniProt Protein Name: Borealin
• UniProt Gene Name: CDCA8
• UniProt Synonym Gene Names: PESCRG3; hDasra-B
• UniProt Entry Name: BOREA_HUMAN

NCBI Description

This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013]

Uniprot Description

Borealin: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Belongs to the borealin family.

Protein type: Cell cycle regulation; Nucleolus

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: intercellular bridge; protein complex; nucleolus; spindle; midbody; nucleus; cytosol; chromosome, pericentric region; chromocenter

Molecular Function: protein binding

Biological Process: cell division; mitotic cell cycle; mitotic metaphase plate congression; chromosome organization and biogenesis