Western Blot (WB)
(Western blot analysis of CDCA8 (arrow) using rabbit polyclonal CDCA8 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDCA8 gene.)
Rabbit anti-Human CDCA8 Polyclonal Antibody | anti-CDCA8 antibody
CDCA8, NT (CDCA8, PESCRG3, Borealin, Dasra-B, Cell division cycle-associated protein 8, Pluripotent embryonic stem cell-related gene 3 protein)
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western blot analysis of CDCA8 (arrow) using rabbit polyclonal CDCA8 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDCA8 gene.)
Western Blot (WB)
(Western blot analysis of CDCA8 (arrow) using rabbit polyclonal CDCA8 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDCA8 gene.)
Immunohistochemistry (IHC)
(CDCA8 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDCA8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CDCA8 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDCA8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CDCA8 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CDCA8 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013]
Uniprot Description
Borealin: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Belongs to the borealin family.
Protein type: Nucleolus; Cell cycle regulation
Chromosomal Location of Human Ortholog: 1p34.3
Cellular Component: intercellular bridge; protein complex; nucleolus; spindle; midbody; cytosol; nucleus; chromosome, pericentric region; chromocenter
Molecular Function: protein binding
Biological Process: cell division; mitotic cell cycle; chromosome organization and biogenesis; mitotic metaphase plate congression
Research Articles on CDCA8
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Product Notes
The CDCA8 cdca8 (Catalog #AAA6005191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDCA8, NT (CDCA8, PESCRG3, Borealin, Dasra-B, Cell division cycle-associated protein 8, Pluripotent embryonic stem cell-related gene 3 protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDCA8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the CDCA8 cdca8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDCA8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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