Rabbit CDCA2 Polyclonal Antibody | anti-CDCA2 antibody

CDCA2 Polyclonal Antibody

Cat. Num. AAA3007508

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE).
• Synonyms: CDCA2; Polyclonal Antibody; CDCA2 Polyclonal Antibody; Cell division cycle-associated protein 2; Recruits PP1 onto mitotic chromatin at anaphase protein; Repo-Man; anti-CDCA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CDCA2 polyclonal antibody detects endogenous levels of CDCA2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1023

• Applicable Applications for anti-CDCA2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 525-569 of Human CDCA2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CDCA2 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:The brain tissue lysate of Mouse (40ug) Lane3:The brain tissue lysate of Rat (40ug))

Related Product Information for anti-CDCA2 antibody

Cell division cycle associated 2 (CDCA2, Repo-Man) is a cell-cycle protein that recruits protein phosphatase 1 (PP1) to mitotic chromatin at anaphase onset, which is essential for cell proliferation. Carboxy-terminal phosphorylation of CDCA2 at Ser893 by Aurora B inhibits the protein and leads to diffuse localization during prometaphase and metaphase. Dephosphorylation of CDCA2 by PP2A is necessary for CDCA2/PP1 complex reformation. The CDCA2/PP1 complex is required for chromatin binding and dephosphorylation of histone H3 at Thr3, Ser10, and Ser28. The CDCA2/PP1 complex is also involved in nuclear envelope reformation during mitotic exit for proper progression through the M/G1 transition. The interaction of CDCA2 with importin beta and Nup153, which is required for nuclear envelope formation, is negatively regulated by CDK phosphorylation of the amino-terminal domain of CDCA2. CDCA2 may play a role in DNA repair as the release of CDCA2 from chromatin at sites of DNA damage promotes the activation of DNA damage response. These results imply that the CDCA2/PP1 complex may play a part in cancer progression. Research studies indicate that CDCA2 may serve as a prognostic marker, as increased CDCA2 expression is seen in a number of cancers, including melanoma, neuroblastoma tumors, squamous cell carcinoma, and synovial sarcomas.

NCBI and Uniprot Product Information

• NCBI GI #: 44681484
• NCBI Accession #: NP_689775.2
• NCBI GenBank Nucleotide #: NP_689775.2
• UniProt Accession #: Q69YH5
• Molecular Weight: ~ 140kDa
• NCBI Official Full Name: cell division cycle-associated protein 2 isoform 1
• UniProt Protein Name: Cell division cycle-associated protein 2
• Protein Family: Cell division cycle-associated protein
• UniProt Gene Name: CDCA2
• UniProt Synonym Gene Names: Repo-Man
• UniProt Entry Name: CDCA2_HUMAN

Uniprot Description

CDCA2: Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nucleoplasm; cytoplasm; chromosome

Biological Process: mitosis; cell division; positive regulation of protein amino acid dephosphorylation; chromosome segregation