Goat CDC42BPA / MRCK Polyclonal Antibody | anti-CDC42BPA antibody

Goat Polyclonal to Human CDC42BPA / MRCK

Cat. Num. AAA247102

• Gene Names: CDC42BPA; MRCK; MRCKA; PK428
• Reactivity: Bat, Gibbon, Bovine, Horse, Human, Monkey, Mouse, Rabbit, Rat; Predicted Reactivity: Chicken (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CDC42BPA / MRCK; Polyclonal Antibody; Goat Polyclonal to Human CDC42BPA / MRCK; Anti-CDC42BPA / MRCK Antibody (N-Terminus) IHC-plus; CDC42BPA; CDC42-binding kinase alpha; DMPK-like alpha; MRCKA; MRCKalpha; KIAA0451; MRCK; PK428; Ser-thr protein kinase PK428; MRCK alpha; Human CDC42BPA; anti-CDC42BPA antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Horse, Human, Monkey, Mouse, Rabbit, Rat; Predicted Reactivity: Chicken (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CDC42BPA / MRCK. This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1719

• Applicable Applications for anti-CDC42BPA antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (3.75 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide SGEVRLRQLEQFI-C from the N-terminus of human CDC42BPA / MRCK (NP_055641.3; NP_003598.2).
• Immunogen Type: Synthetic peptide
• Immunogen: CDC42BPA / MRCK antibody was raised against synthetic peptide SGEVRLRQLEQFI-C from the N-terminus of human CDC42BPA / MRCK (NP_055641.3; NP_003598.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Horse, Rabbit, Opossum, Platypus, Lizard (100%); Chicken (92%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CDC42BPA / MRCK antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Related Product Information for anti-CDC42BPA antibody

MRCK alpha belongs to the DMPK subfamily. MRCK alpha is a downstream effector of Cdc42 and Rac where it is involved in actin cytoskeletal reorganization and neurite outgrowth.

Product Categories/Family for anti-CDC42BPA antibody

Family: Protein Kinase; Subfamily: DMPK

NCBI and Uniprot Product Information

• NCBI GI #: 30089962
• NCBI GeneID: 8476
• NCBI Accession #: NP_003598.2
• NCBI GenBank Nucleotide #: NM_003607.3
• UniProt Accession #: Q5VT25; O75039; Q59GZ1; Q5H9N9; Q5T797; Q5VT26; Q5VT27; Q86XX2; Q86XX3; Q99646
• Molecular Weight: 202,812 Da
• NCBI Official Full Name: serine/threonine-protein kinase MRCK alpha isoform B
• NCBI Official Synonym Full Names: CDC42 binding protein kinase alpha (DMPK-like)
• NCBI Official Symbol: CDC42BPA
• NCBI Official Synonym Symbols: MRCK; MRCKA; PK428
• NCBI Protein Information: serine/threonine-protein kinase MRCK alpha; CDC42BPA; DMPK-like alpha; ser-thr protein kinase PK428; CDC42 binidng protein kinase beta; myotonic dystrophy protein kinase-like alpha; CDC42-binding protein kinase alpha (DMPK-like); myotonic dystrophy kinase-related CDC42-binding kinase alpha; myotonic dystrophy kinase-related CDC42-binding protein kinase alpha; ser-thr protein kinase related to the myotonic dystrophy protein kinase
• UniProt Protein Name: Serine/threonine-protein kinase MRCK alpha
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: CDC42BPA
• UniProt Synonym Gene Names: MRCK alpha; Myotonic dystrophy protein kinase-like alpha
• UniProt Entry Name: MRCKA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MRCKA: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; AGC group; DMPK family; GEK subfamily

Chromosomal Location of Human Ortholog: 1q42.11

Cellular Component: cytoplasm; leading edge; actomyosin; intercellular junction

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding

Biological Process: cell migration; regulation of catalytic activity; actomyosin structure organization and biogenesis; actin cytoskeleton reorganization; microtubule cytoskeleton organization and biogenesis; protein amino acid phosphorylation; nuclear migration