Rabbit anti-Human, Mouse CDC42BPA Polyclonal Antibody | anti-CDC42BPA antibody

CDC42BPA Polyclonal Antibody

Cat. Num. AAA3007057

• Gene Names: CDC42BPA; MRCK; MRCKA; PK428
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CDC42BPA; Polyclonal Antibody; CDC42BPA Polyclonal Antibody; Serine/threonine-protein kinase MRCK alpha; CDC42-binding protein kinase alpha; DMPK-like alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; MRCK alpha; Myotonic dystrophy protein kinase-like alpha; KIAA0451; anti-CDC42BPA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CDC42BPA polyclonal antibody detects endogenous levels of CDC42BPA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1719

• Applicable Applications for anti-CDC42BPA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human CDC42BPA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CDC42BPA pAb at 1:500 dilutionLane1:Panc1 whole cell lysate (40ug) Lane2: U-87MG whole cell lysate (40ug))

Related Product Information for anti-CDC42BPA antibody

Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Myotonic dystrophy kinase-related Cdc42-binding (DMPK-like) kinases-a and b (MRCK-a, b) contain a cysteine-rich motif and a putative pleckstrin homology domain. MRCKs can phosphorylate nonmuscle myosin light chain and influences actin-myosin contractility. MRCK-a can phosphorylate and activate LIM kinases downstream of Cdc42, which leads to inactivation of ADF/cofilin and to Actin cytoskeletal reorganization. MRCK-a can also influence neurite outgrowth promoted by Cdc42 and Rac.

NCBI and Uniprot Product Information

• NCBI GI #: 30089962
• NCBI GeneID: 8476
• NCBI Accession #: NP_003598.2
• NCBI GenBank Nucleotide #: NP_003598.2
• UniProt Accession #: Q5VT25
• Molecular Weight: ~ 186kDa
• NCBI Official Full Name: serine/threonine-protein kinase MRCK alpha isoform B
• NCBI Official Synonym Full Names: CDC42 binding protein kinase alpha
• NCBI Official Symbol: CDC42BPA
• NCBI Official Synonym Symbols: MRCK; MRCKA; PK428
• NCBI Protein Information: serine/threonine-protein kinase MRCK alpha
• UniProt Protein Name: Serine/threonine-protein kinase MRCK alpha
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: CDC42BPA
• UniProt Synonym Gene Names: KIAA0451; MRCK alpha
• UniProt Entry Name: MRCKA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described. [provided by RefSeq, Sep 2018]

Uniprot Description

MRCKA: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); AGC group; DMPK family; GEK subfamily

Chromosomal Location of Human Ortholog: 1q42.11

Cellular Component: cytoplasm; leading edge; actomyosin; intercellular junction

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding

Biological Process: cell migration; regulation of catalytic activity; actomyosin structure organization and biogenesis; actin cytoskeleton reorganization; microtubule cytoskeleton organization and biogenesis; nuclear migration; protein amino acid phosphorylation