Western Blot (WB)
(Western blot analysis of extracts from Jurkat/COLO205 cells, using MRCKa antibody.)
Rabbit CDC42BPA Polyclonal Antibody | anti-CDC42BPA antibody
CDC42BPA Antibody
Gene Names
CDC42BPA; MRCK; MRCKA; PK428
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CDC42BPA; Polyclonal Antibody; CDC42BPA Antibody; A930014J19Rik; CDC42 binding protein kinase alpha (DMPK like); CDC42 binding protein kinase alpha; CDC42 binding protein kinase alpha dmpk like; CDC42 binding protein kinase beta; CDC42-binding protein kinase alpha; DKFZp686L1738; DKFZp686P1738; DMPK like; DMPK-like alpha; FLJ23347; KIAA0451; MGC189823; MRCK alpha; mrck; mrcka; MRCKA_HUMAN; Myotonic dystrophy kinase related CDC42 binding protein kinase alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; Myotonic dystrophy protein kinase-like alpha; OTTHUMP00000035727; OTTHUMP00000035728; OTTHUMP00000035729; OTTHUMP00000035730; pk428; RP5-1087E8.4; Ser thr protein kinase PK428; Ser thr protein kinase related to the myotonic dystrophy protein kinase; Serine/threonine protein kinase MRCK alpha; Serine/threonine-protein kinase MRCK alpha; anti-CDC42BPA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CDC42BPA antibody detects endogenous levels of total CDC42BPA
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1719
Applicable Applications for anti-CDC42BPA antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
Tissue Specificity
Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Jurkat/COLO205 cells, using MRCKa antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat/COLO205 cells, using MRCKa antibody.)
Related Product Information for anti-CDC42BPA antibody
Function: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed:15723050, PubMed:9418861, PubMed:9092543). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160). Phosphorylates: PPP1R12A, LIMK1 and LIMK2 (PubMed:11340065, PubMed:11399775). May play a role in TFRC-mediated iron uptake (PubMed:20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).
Subunit Structure: Homodimer and homotetramer via the coiled coil regions (PubMed:11283256). Interacts tightly with GTP-bound but not GDP-bound CDC42 (PubMed:9418861). Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPA and MYO18A. LURAP1 binding results in activation of CDC42BPA by abolition of its negative autoregulation (PubMed:18854160). Interacts with LURAP1. Interacts (via AGC-kinase C-terminal domain) with FAM89B/LRAP25 (via LRR repeat). Forms a tripartite complex with FAM89B/LRAP25 and LIMK1 (By similarity).
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
Subunit Structure: Homodimer and homotetramer via the coiled coil regions (PubMed:11283256). Interacts tightly with GTP-bound but not GDP-bound CDC42 (PubMed:9418861). Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPA and MYO18A. LURAP1 binding results in activation of CDC42BPA by abolition of its negative autoregulation (PubMed:18854160). Interacts with LURAP1. Interacts (via AGC-kinase C-terminal domain) with FAM89B/LRAP25 (via LRR repeat). Forms a tripartite complex with FAM89B/LRAP25 and LIMK1 (By similarity).
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 97 kD
Predicted: 198 kDa
Predicted: 198 kDa
NCBI Official Full Name
serine/threonine-protein kinase MRCK alpha isoform B
NCBI Official Synonym Full Names
CDC42 binding protein kinase alpha
NCBI Official Symbol
CDC42BPA
NCBI Official Synonym Symbols
MRCK; MRCKA; PK428
NCBI Protein Information
serine/threonine-protein kinase MRCK alpha
UniProt Protein Name
Serine/threonine-protein kinase MRCK alpha
Protein Family
UniProt Gene Name
CDC42BPA
UniProt Synonym Gene Names
MRCK alpha; Myotonic dystrophy protein kinase-like alpha
NCBI Description
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described. [provided by RefSeq, Sep 2018]
Uniprot Description
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed:15723050, PubMed:9418861, PubMed:9092543). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160). Phosphorylates: PPP1R12A, LIMK1 and LIMK2 (PubMed:11340065, PubMed:11399775). May play a role in TFRC-mediated iron uptake (PubMed:20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation ().
Research Articles on CDC42BPA
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Product Notes
The CDC42BPA cdc42bpa (Catalog #AAA9606240) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC42BPA Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDC42BPA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the CDC42BPA cdc42bpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC42BPA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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