Rabbit CDC27 Polyclonal Antibody | anti-CDC27 antibody

Rabbit Polyclonal to Human CDC27

Cat. Num. AAA243778

• Gene Names: CDC27; APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CDC27; Polyclonal Antibody; Rabbit Polyclonal to Human CDC27; Anti-CDC27 Antibody (C-Terminus) IHC-plus; ANAPC3; APC3; CDC27 homolog; Cell division cycle 27; D0S1430E; CDC27Hs; D17S978E; H-NUC; Nuc2 homolog; HNUC; NUC2; Human CDC27; anti-CDC27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human CDC27
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 824

• Applicable Applications for anti-CDC27 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: APC3 antibody can be used for detection of APC3 by Western blot at 1-2 ug/ml.
• Target Species: Human
• Immunogen Description: A 19 amino acid peptide near the carboxy terminus of human APC3.
• Immunogen Type: Synthetic peptide
• Immunogen: CDC27 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APC3.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-CDC27 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CDC27 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 167466175
• NCBI GeneID: 996
• NCBI Accession #: NP_001247.3
• NCBI GenBank Nucleotide #: NM_001256.4
• UniProt Accession #: P30260; Q16349; Q96F35; G3V1C4
• Molecular Weight: 92,612 Da
• NCBI Official Full Name: cell division cycle protein 27 homolog isoform 2
• NCBI Official Synonym Full Names: cell division cycle 27
• NCBI Official Symbol: CDC27
• NCBI Official Synonym Symbols: APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E
• NCBI Protein Information: cell division cycle protein 27 homolog; nuc2 homolog; cell division cycle 27 homolog; anaphase promoting complex subunit 3; anaphase-promoting complex, protein 3
• UniProt Protein Name: Cell division cycle protein 27 homolog
• Protein Family: Cell division cycle protein
• UniProt Gene Name: CDC27
• UniProt Synonym Gene Names: ANAPC3; D0S1430E; D17S978E; APC3; CDC27Hs
• UniProt Entry Name: CDC27_HUMAN

NCBI Description

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]

Uniprot Description

CDC27: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C is composed of at least 12 subunits. Interacts with RB. Interacts with FAM168B/MANI. Belongs to the APC3/CDC27 family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; centrosome; anaphase-promoting complex; spindle microtubule; cytoplasm; spindle; cytosol; nucleus

Molecular Function: protein binding; protein phosphatase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell proliferation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; mitotic metaphase/anaphase transition