Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using CDC25C (Phospho-Ser198) Antibody. The picture on the right is blocked with the phospho peptide.)
Rabbit anti-Human CDC25C Polyclonal Antibody | anti-CDC25C antibody
CDC25C (Phospho-Ser198) Antibody
Gene Names
CDC25C; CDC25; PPP1R60
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
CDC25C; Polyclonal Antibody; CDC25C (Phospho-Ser198) Antibody; CDC25M1; dual specificity phosphatase Cdc25C; M-phase inducer phosphatase 3; MPI1B; MPIP3; anti-CDC25C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
CDC25C (Phospho-Ser198) Antibody detects endogenous levels of CDC25C only when phosphorylated at Ser198.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
400
Applicable Applications for anti-CDC25C antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50~1:100
ELISA: 1:1000
ELISA: 1:1000
Target Modification
Phospho
Modification Sites
H:S198
Immunogen
The antiserum was produced against synthesized peptide derived from human CDC25C around the phosphorylation site of Ser198.
Immunogen Range
164-213
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using CDC25C (Phospho-Ser198) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using CDC25C (Phospho-Ser198) Antibody. The picture on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53 kDa
NCBI Official Full Name
M-phase inducer phosphatase 3 isoform a
NCBI Official Synonym Full Names
cell division cycle 25C
NCBI Official Symbol
CDC25C
NCBI Official Synonym Symbols
CDC25; PPP1R60
NCBI Protein Information
M-phase inducer phosphatase 3
UniProt Protein Name
M-phase inducer phosphatase 3
UniProt Gene Name
CDC25C
NCBI Description
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]
Uniprot Description
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Research Articles on CDC25C
1. Mdm2 overexpression and Cdc25C downregulation delay cell cycle progression through the G2/M phase.
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Product Notes
The CDC25C cdc25c (Catalog #AAA9503353) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC25C (Phospho-Ser198) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC25C can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50~1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the CDC25C cdc25c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC25C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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