Western Blot (WB)
(HSD17B4 Antibody (Center) western blot analysis in MCF-7,ZR-75-1,HepG2 cell line lysates (35ug/lane).This demonstrates the HSD17B4 antibody detected the HSD17B4 protein (arrow).)
Rabbit anti-Human HSD17B4 Polyclonal Antibody | anti-HSD17B4 antibody
HSD17B4, ID (HSD17B4, EDH17B4, Peroxisomal multifunctional enzyme type 2, 17-beta-hydroxysteroid dehydrogenase 4, D-bifunctional protein, Multifunctional protein 2, (3R)-hydroxyacyl-CoA dehydrogenase, Enoyl-CoA hydratase 2, 3-alpha,7-alpha,12-alpha-trihyd
Gene Names
HSD17B4; DBP; MFE-2; MPF-2; PRLTS1; SDR8C1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HSD17B4; Polyclonal Antibody; ID (HSD17B4; EDH17B4; Peroxisomal multifunctional enzyme type 2; 17-beta-hydroxysteroid dehydrogenase 4; D-bifunctional protein; Multifunctional protein 2; (3R)-hydroxyacyl-CoA dehydrogenase; Enoyl-CoA hydratase 2; 3-alpha; 7-alpha; 12-alpha-trihyd; 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; anti-HSD17B4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-HSD17B4 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
HSD17B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 340-370 amino acids from the Central region of human HSD17B4.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(HSD17B4 Antibody (Center) western blot analysis in MCF-7,ZR-75-1,HepG2 cell line lysates (35ug/lane).This demonstrates the HSD17B4 antibody detected the HSD17B4 protein (arrow).)
Western Blot (WB)
(HSD17B4 Antibody (Center) western blot analysis in MCF-7,ZR-75-1,HepG2 cell line lysates (35ug/lane).This demonstrates the HSD17B4 antibody detected the HSD17B4 protein (arrow).)
Immunohistochemistry (IHC)
(HSD17B4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HSD17B4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(HSD17B4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HSD17B4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-HSD17B4 antibody
The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq].
Product Categories/Family for anti-HSD17B4 antibody
References
Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Kashiwayama, Y., et al. J. Biol. Chem. 285(34):26315-26325(2010). Pierce, S.B., et al. Am. J. Hum. Genet. 87(2):282-288(2010). Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77,870 Da
NCBI Official Full Name
peroxisomal multifunctional enzyme type 2 isoform 2
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 4
NCBI Official Symbol
HSD17B4
NCBI Official Synonym Symbols
DBP; MFE-2; MPF-2; PRLTS1; SDR8C1
NCBI Protein Information
peroxisomal multifunctional enzyme type 2
UniProt Protein Name
Peroxisomal multifunctional enzyme type 2
UniProt Gene Name
HSD17B4
UniProt Synonym Gene Names
EDH17B4; MFE-2; 17-beta-HSD 4; DBP; MPF-2
UniProt Entry Name
DHB4_HUMAN
NCBI Description
The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
Research Articles on HSD17B4
1. Structural MFE-2 instability is the molecular basis of D-bifunctional protein deficiency type III.
Similar Products
Product Notes
The HSD17B4 hsd17b4 (Catalog #AAA6308507) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD17B4, ID (HSD17B4, EDH17B4, Peroxisomal multifunctional enzyme type 2, 17-beta-hydroxysteroid dehydrogenase 4, D-bifunctional protein, Multifunctional protein 2, (3R)-hydroxyacyl-CoA dehydrogenase, Enoyl-CoA hydratase 2, 3-alpha,7-alpha,12-alpha-trihyd reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSD17B4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HSD17B4 hsd17b4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD17B4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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