Western Blot (WB)
(Anti-Cdc25B antibody, MBS175687, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: Rat Lung Tissue Lysate )
Rabbit anti-Human, Rat Cdc25B Polyclonal Antibody | anti-CDC25B antibody
Anti-Cdc25B antibody
IHC-p: Concentration: 0.5-1ug/mL, Tested Species: Human , Rat; Antigen Retrieval: By Heat
Tested Species: In house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, Ph6.0, for 20mins is required for the staining of formailin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
QC lab provides a series of assays reacted with primary antibodies . Antibodies can be supported by chemiluminescence kit MBS716460 in WB, supported by MBS746451 in IH(P).
Western Blot (WB)
(Anti-Cdc25B antibody, MBS175687, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: Rat Lung Tissue Lysate )
Western Blot (WB)
(Anti-Cdc25B antibody, MBS175687, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: Rat Lung Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-Cdc25B antibody, MBS175687, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-Cdc25B antibody, MBS175687, IHC(P)IHC(P): Human Lung Cancer Tissue )
NCBI and Uniprot Product Information
NCBI Description
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]
Uniprot Description
CDC25B: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.
Protein type: Nuclear receptor co-regulator; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol
Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding
Biological Process: positive regulation of cytokinesis; mitosis; cell division; positive regulation of protein kinase activity; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; positive regulation of mitotic cell cycle; oocyte maturation; protein amino acid phosphorylation; female meiosis I
Research Articles on CDC25B
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Product Notes
The CDC25B cdc25b (Catalog #AAA175687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cdc25B antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cdc25B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: Concentration: 0.1-0.5ug/mL, Tested Species: Rat, Concluded Species: Human IHC-p: Concentration: 0.5-1ug/mL, Tested Species: Human, Rat; Antigen Retrieval: By Heat Tested Species: In house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, Ph6.0, for 20mins is required for the staining of formailin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end user. QC lab provides a series of assays reacted with primary antibodies. Antibodies can be supported by chemiluminescence kit MBS716460 in WB, supported by MBS746451 in IH(P). Researchers should empirically determine the suitability of the CDC25B cdc25b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdc25B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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