Rabbit anti-Human CDC25A Polyclonal Antibody

CDC25A, phosphorylated (Ser278) (M-phase Inducer Phosphatase 1, Dual Specificity Phosphatase Cdc25A)

Cat. Num. AAA628707

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CDC25A; Polyclonal Antibody; phosphorylated (Ser278) (M-phase Inducer Phosphatase 1; Dual Specificity Phosphatase Cdc25A); Anti -CDC25A; anti-CDC25A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Cdc25A phosphorylated at Ser278.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CDC25A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser278 of human Cdc25A (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Phospho-CDC25A-S278 Pab is used in Western blot to detect Phospho-CDC25A-S278 in mouse heart tissue lysate)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-CDC25A antibody

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CDC25A antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 68135376
• NCBI Official Full Name: CDC25A
• Protein Family: M-phase inducer phosphatase

NCBI Description

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: cell proliferation; mitosis; response to radiation; cell division; regulation of cell cycle; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; G2/M transition of mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle