Rabbit Cdc20 Polyclonal Antibody | anti-Cdc20 antibody

Cdc20 (Q105) Polyclonal Antibody

Cat. Num. AAA3002283

• Gene Names: CDC20; CDC20A; p55CDC; bA276H19.3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cdc20; Polyclonal Antibody; Cdc20 (Q105) Polyclonal Antibody; Cell division cycle protein 20 homolog; p55CDC; CDC20; anti-Cdc20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cdc20 (Q105) polyclonal antibody detects endogenous levels of Cdc20 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 499

• Applicable Applications for anti-Cdc20 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human Cdc20.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cdc20 (Q105) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:Rat liver tissue lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Cdc20 (Q105) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Cdc20 (Q105) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of Cdc20 (Q105) pAb at 1:500 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:The Heart tissue lysate of Mouse (40ug) Lane4:The Heart tissue lysate of Rat (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of Cdc20 (Q105) pAb pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Prostate tissue lysate of Rat (40ug))

Related Product Information for anti-Cdc20 antibody

Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family. Exit from mitosis and initiation of the next cell cycle requires a complex of proteins designated the anaphase-promoting complex (APC). This complex consists of two proteins, Cdc16 and Cdc27 (also referred to as Snb1), which are involved in limiting DNA replication to once per cell cycle. Cdc23, another component of the APC, is required for both entering and exiting anaphase, and is important for the proper separation of sister chromatids. The APC is thought to be stabilized by Cdc26 (also known as Scd26). In addition to these APC proteins, Cdc5 is also required for completion of mitosis. In contrast, Cdc20 acts as a DNA-damage induced checkpoint, preventing mitosis when DNA damage has occurred..

NCBI and Uniprot Product Information

• NCBI GI #: 118402582
• NCBI GeneID: 991
• NCBI Accession #: NP_001246.2
• NCBI GenBank Nucleotide #: NP_001246.2
• UniProt Accession #: Q12834
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: cell division cycle protein 20 homolog
• NCBI Official Synonym Full Names: cell division cycle 20
• NCBI Official Symbol: CDC20
• NCBI Official Synonym Symbols: CDC20A; p55CDC; bA276H19.3
• NCBI Protein Information: cell division cycle protein 20 homolog
• UniProt Protein Name: Cell division cycle protein 20 homolog
• Protein Family: Cell division cycle protein
• UniProt Gene Name: CDC20
• UniProt Entry Name: CDC20_HUMAN

NCBI Description

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC20: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with isoform 1 of NEK2. Belongs to the WD repeat CDC20/Fizzy family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleoplasm; spindle pole; centrosome; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; spindle; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein binding; enzyme binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of synaptic plasticity; regulation of meiosis; regulation of dendrite development; protein ubiquitination; cell cycle; anaphase-promoting complex activation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of cell proliferation; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle