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Western Blot (WB) (Western blot analysis of anti-THY1 (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2050a) in T47D cell line lysate. THY1 (N-term)(arrow) was detected using the purified Pab.)

Rabbit anti-Human CD90 Polyclonal Antibody | anti-CD90 antibody

CD90, NT (Thy-1 Membrane Glycoprotein, Thy-1 Antigen, THY1, CDw90) (PE)

Gene Names
THY1; CD90; CDw90
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
CD90; Polyclonal Antibody; NT (Thy-1 Membrane Glycoprotein; Thy-1 Antigen; THY1; CDw90) (PE); anti-CD90 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human THY1.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CD90 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human THY1 (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-THY1 (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2050a) in T47D cell line lysate. THY1 (N-term)(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-THY1 (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2050a) in T47D cell line lysate. THY1 (N-term)(arrow) was detected using the purified Pab.)

Western Blot (WB)

(The anti-THY1 (N-term) Pab is used in Western blot to detect THY1 in HL60 cell lysate.)

Western Blot (WB) (The anti-THY1 (N-term) Pab is used in Western blot to detect THY1 in HL60 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with C2441-04D which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with C2441-04D which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody C2441-04D), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.. HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody C2441-04D), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.. HC = hepatocarcinoma.)
Product Categories/Family for anti-CD90 antibody
References
1. Koumas, L., et al., Am. J. Pathol. 163(4):1291-1300 (2003). 2. Abeysinghe, H.R., et al., Cancer Genet. Cytogenet. 143(2):125-132 (2003). 3. Saalbach, A., et al., Microvasc. Res. 64(1):86-93 (2002). 4. Henniker, A.J., J. Biol. Regul. Homeost. Agents 15(4):392-393 (2001). 5. Ye, Z., et al., Biochem. Biophys. Res. Commun. 275(1):223-227 (2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13.3kDa (117aa) 13.5-28kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
thy-1 membrane glycoprotein preproprotein
NCBI Official Synonym Full Names
Thy-1 cell surface antigen
NCBI Official Symbol
THY1
NCBI Official Synonym Symbols
CD90; CDw90
NCBI Protein Information
thy-1 membrane glycoprotein
UniProt Protein Name
Thy-1 membrane glycoprotein
UniProt Gene Name
THY1
UniProt Entry Name
THY1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: anchored to external side of plasma membrane; focal adhesion; growth cone; endoplasmic reticulum; integral to plasma membrane; dendrite; apical plasma membrane; plasma membrane; cytosol; lipid raft; external side of plasma membrane

Molecular Function: integrin binding; protein binding; protein kinase binding

Biological Process: focal adhesion formation; cell-cell adhesion; retinal cone cell development; negative regulation of T cell receptor signaling pathway; negative regulation of protein kinase activity; negative regulation of axonogenesis; cytoskeleton organization and biogenesis; angiogenesis; positive regulation of T cell activation; positive regulation of release of sequestered calcium ion into cytosol; T cell receptor signaling pathway; negative regulation of cell migration; positive regulation of GTPase activity

Research Articles on CD90

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Product Notes

The CD90 thy1 (Catalog #AAA6468158) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD90, NT (Thy-1 Membrane Glycoprotein, Thy-1 Antigen, THY1, CDw90) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD90 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD90 thy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD90, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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