Testing Data
(Fig: Western blot analysis on HepG2 cell lysates using anti-CD9 polyclonal antibody)
CD9 Polyclonal Antibody | anti-CD9 antibody
CD9 Antibody
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Mouse, Rat, Human
Applications
Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity purified
Synonyms
CD9; Polyclonal Antibody; CD9 Antibody; anti-CD9 antibody
Reactivity
Mouse, Rat, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of CD9.
Purity/Purification
Affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 25% Glycerol.
Sequence Length
159
Applicable Applications for anti-CD9 antibody
Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1,000
ICC: 1:100
ICC: 1:100
Preservative
0.05% Sodium Azide.
Cellular Localization
Cell Membrane
Positive Control
HepG2
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.
Testing Data
(Fig: Western blot analysis on HepG2 cell lysates using anti-CD9 polyclonal antibody)
Testing Data
(Fig: Western blot analysis on HepG2 cell lysates using anti-CD9 polyclonal antibody)
Related Product Information for anti-CD9 antibody
CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. Protein exists in three forms, and is known to carry covalently linked fatty acids. It is involved in platelet activation, aggregation, in cell adhesion, cell motility and tumor metastasis. It regulates paranodal junction formation, and is also required for gamete fusion.
References
1. Higashihara M., Takahata K., Yatomi Y., Nakahara K., Kurokawa K.; "Purification and partial characterization of CD9 antigen of human platelets."; FEBS Lett. 264:270-274(1990). 2. Ikeyama S., Koyama M., Yamaoko M., Sasada R., Miyake M.; "Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA."; J. Exp. Med. 177:1231-1237(1993). 3. Rubinstein E., Benoit P., Billard M., Plaisance S., Prenant M., Uzan G., Boucheix C.; "Organization of the human CD9 gene."; Genomics 16:132-138(1993).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~43kDa
NCBI Official Full Name
CD9 antigen isoform 2
NCBI Official Synonym Full Names
CD9 molecule
NCBI Official Symbol
CD9
NCBI Official Synonym Symbols
MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
NCBI Protein Information
CD9 antigen
UniProt Protein Name
CD9 antigen
Protein Family
UniProt Gene Name
CD9
UniProt Synonym Gene Names
MIC3; TSPAN29; MRP-1; Tspan-29
NCBI Description
This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
Uniprot Description
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Research Articles on CD9
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Product Notes
The CD9 cd9 (Catalog #AAA857046) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CD9 Antibody reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:500-1:1,000 ICC: 1:100. Researchers should empirically determine the suitability of the CD9 cd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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