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Western Blot (WB) (Western blot analysis of extracts of mouse kidney with CD9 Polyclonal Antibody)

Rabbit anti-Human, Mouse CD9 Polyclonal Antibody | anti-CD9 antibody

CD9 Antibody

Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Antigen affinity purification
Synonyms
CD9; Polyclonal Antibody; CD9 Antibody; BTCC-1; DRAP-27; MIC3; MRP-1; TSPAN-29; TSPAN29; anti-CD9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Sequence Length
228
Applicable Applications for anti-CD9 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human CD9
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7639 / sc-9148
Preparation and Storage
Store at -20 degree C. Avoid freeze

Western Blot (WB)

(Western blot analysis of extracts of mouse kidney with CD9 Polyclonal Antibody)

Western Blot (WB) (Western blot analysis of extracts of mouse kidney with CD9 Polyclonal Antibody)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CD9 antibody. For SW480, the Observed MW is 25kDa. For A549, the Observed MW is 35kDa, because there are two glycosylated modification sites for the target protein)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CD9 antibody. For SW480, the Observed MW is 25kDa. For A549, the Observed MW is 35kDa, because there are two glycosylated modification sites for the target protein)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human endometrial cancer using CD9 antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human endometrial cancer using CD9 antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human spleen hematoma using CD9 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human spleen hematoma using CD9 antibody at dilution of 1:100 (400x lens).)
Related Product Information for anti-CD9 antibody
This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
928
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 25kDa
Observed MW: 25kDa
NCBI Official Full Name
CD9 antigen
NCBI Official Synonym Full Names
CD9 molecule
NCBI Official Symbol
CD9
NCBI Official Synonym Symbols
MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
NCBI Protein Information
CD9 antigen; 5H9 antigen; BA-2/p24 antigen; CD9 antigen (p24); cell growth-inhibiting gene 2 protein; leukocyte antigen MIC3; motility related protein-1; tetraspanin-29
UniProt Protein Name
CD9 antigen
Protein Family
UniProt Gene Name
CD9
UniProt Synonym Gene Names
MIC3; TSPAN29; MRP-1; Tspan-29
UniProt Entry Name
CD9_HUMAN

NCBI Description

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Uniprot Description

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; platelet alpha granule membrane; focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle; external side of plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: platelet activation; fusion of sperm to egg plasma membrane; pathogenesis; multicellular organism reproduction; paranodal junction assembly; negative regulation of cell proliferation; platelet degranulation; single fertilization; brain development; blood coagulation; oligodendrocyte development; cell adhesion; cell motility; response to water deprivation

Research Articles on CD9

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Product Notes

The CD9 cd9 (Catalog #AAA2524710) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD9 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the CD9 cd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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