Rabbit anti-Human CD8A Polyclonal Antibody | anti-CD8A antibody

CD8A, CT (T-cell Surface Glycoprotein CD8 alpha Chain, T-lymphocyte Differentiation Antigen T8/Leu-2, MAL)

Cat. Num. AAA6008533

• Gene Names: CD8A; CD8; MAL; p32; Leu2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CD8A; Polyclonal Antibody; CT (T-cell Surface Glycoprotein CD8 alpha Chain; T-lymphocyte Differentiation Antigen T8/Leu-2; MAL); Anti -CD8A; anti-CD8A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human CD8A Molecule.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide.

• Applicable Applications for anti-CD8A antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 13aa from human CD8A Molecule at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Human tonsil stained with MBS6008533.)

Immunohistochemistry (IHC)

(Human Thymus (formalin-fixed, paraffin-embedded) stained with CD8A antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-CD8A antibody

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified.

Product Categories/Family for anti-CD8A antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 19344022
• NCBI GeneID: 925
• UniProt Accession #: P01732; Q13970; Q4ZG17; D6W5M8
• Molecular Weight: 25,729 Da
• NCBI Official Full Name: CD8a molecule
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; MAL; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL
• UniProt Entry Name: CD8A_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: T cell receptor complex; integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; MHC class I protein binding; coreceptor activity; protein kinase binding

Biological Process: antigen processing and presentation; regulation of immune response; T cell activation; T cell mediated immunity; positive regulation of calcium-mediated signaling; immune response; cytotoxic T cell differentiation; defense response to virus; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Cd8 Deficiency, Familial