Rabbit anti-Human CD8a Polyclonal Antibody | anti-CD8a antibody

CD8a (T-cell Surface Glycoprotein CD8 alpha Chain) (FITC)

Cat. Num. AAA6403345

• Gene Names: CD8A; CD8; p32; Leu2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD8a; Polyclonal Antibody; CD8a (T-cell Surface Glycoprotein CD8 alpha Chain) (FITC); anti-CD8a antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD8a.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-CD8a antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB), FLISA, Immunohistochemistry (IHC)
• Application Notes: FC/FACS: 1-2ug/10e6 cells; IF: 1-2ug/ml; IP: 1-2ug/500ug protein lysate; WB: 1-2ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to aa200-250 from human CD8a.
• Conjugate: FITC
• Positive Control: HuT78 or hPBL. Tonsil
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human tonsil tissue stained with CD8a antibody (1:200), peroxidase-conjugate and DAB chromogen.)

Product Categories/Family for anti-CD8a antibody

Antibodies; CD Markers

References

1. Mason DY, et. al. Journal of Clinical Pathology, 1992, 45(12):1084-8.

NCBI and Uniprot Product Information

• NCBI GI #: 225007536
• NCBI GeneID: 925
• NCBI Accession #: NP_001139345
• NCBI GenBank Nucleotide #: NM_001145873
• UniProt Accession #: P01732
• Molecular Weight: 24kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL
• UniProt Entry Name: CD8A_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]