Western Blot (WB)
(Western Blot detection against Immunogen (46.9kD).)
Mouse anti-Human DNAH14 Monoclonal Antibody | anti-DNAH14 antibody
DNAH14 (Dynein Heavy Chain 14, Axonemal, Axonemal beta Dynein Heavy Chain 14, C1orf67 Ciliary Dynein Heavy Chain 14, MGC149665, MGC149666, MGC27277, MGC51214)
Gene Names
DNAH14; HL18; HL-18; C1orf67; Dnahc14
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
DNAH14; Monoclonal Antibody; DNAH14 (Dynein Heavy Chain 14; Axonemal; Axonemal beta Dynein Heavy Chain 14; C1orf67 Ciliary Dynein Heavy Chain 14; MGC149665; MGC149666; MGC27277; MGC51214); Anti -DNAH14 (Dynein Heavy Chain 14; anti-DNAH14 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3C12
Specificity
Recognizes human MGC27277.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MDKEETKTKPRLLRYEEKKYEDVKPLETQPAEIAEKETLEYKTVRTFSESLKSEKTEDYLRESIIQQHMVSPEPASLKEKGKSRRKKDQTHACPNVRKARPVSYDRTEPKDDDVIRNIIRLREKLGWQTILPQHSLKYGSSKIAIQKITLKKPLEDDGEFVYCLPRKSPKSLYNPYDLQVINIVGSVKE
Applicable Applications for anti-DNAH14 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Immunogen
Full length recombinant protein corresponding to aa1-190 from human MGC27277 (AAH42869) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (46.9kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (46.9kD).)
Testing Data
(Detection limit for recombinant GST tagged C1orf67 is 1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged C1orf67 is 1ng/ml as a capture antibody.)
Related Product Information for anti-DNAH14 antibody
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly.
Product Categories/Family for anti-DNAH14 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
399,895 Da
NCBI Official Full Name
DNAH14 protein
NCBI Official Synonym Full Names
dynein, axonemal, heavy chain 14
NCBI Official Symbol
DNAH14
NCBI Official Synonym Symbols
HL18; HL-18; C1orf67; Dnahc14
NCBI Protein Information
dynein heavy chain 14, axonemal; ciliary dynein heavy chain 14; axonemal beta dynein heavy chain 14; dynein, axonemal, heavy polypeptide 14
UniProt Protein Name
Dynein heavy chain 14, axonemal
Protein Family
UniProt Gene Name
DNAH14
UniProt Synonym Gene Names
C1orf67
UniProt Entry Name
DYH14_HUMAN
NCBI Description
Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains. Two major classes of dyneins, axonemal and cytoplasmic, have been identified. DNAH14 is an axonemal dynein heavy chain (DHC) (Vaughan et al., 1996 [PubMed 8812413]).[supplied by OMIM, Mar 2008]
Uniprot Description
DNAH14: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly. Belongs to the dynein heavy chain family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q42.12
Cellular Component: dynein complex; microtubule; cytoplasm; cilium
Molecular Function: microtubule motor activity; ATP binding
Biological Process: metabolic process; microtubule-based movement
Similar Products
Product Notes
The DNAH14 dnah14 (Catalog #AAA6010004) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DNAH14 (Dynein Heavy Chain 14, Axonemal, Axonemal beta Dynein Heavy Chain 14, C1orf67 Ciliary Dynein Heavy Chain 14, MGC149665, MGC149666, MGC27277, MGC51214) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAH14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the DNAH14 dnah14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MDKEETKTKP RLLRYEEKKY EDVKPLETQP AEIAEKETLE YKTVRTFSES LKSEKTEDYL RESIIQQHMV SPEPASLKEK GKSRRKKDQT HACPNVRKAR PVSYDRTEPK DDDVIRNIIR LREKLGWQTI LPQHSLKYGS SKIAIQKITL KKPLEDDGEF VYCLPRKSPK SLYNPYDLQV INIVGSVKE. It is sometimes possible for the material contained within the vial of "DNAH14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
