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Western Blot (WB) (Western blot analysis of SELL Antibody (C-term) in Jurkat cell line lysates (35ug/lane).SELL (arrow) was detected using the purified Pab.)

Rabbit anti-Human CD62L Polyclonal Antibody | anti-CD62L antibody

CD62L, CT (L-selectin, Lymph Node Homing Receptor, Leukocyte Adhesion Molecule 1, LAM-1, Leukocyte Surface Antigen Leu-8, TQ1, gp90-MEL, Leukocyte-endothelial Cell Adhesion Molecule 1, LECAM1, CD62 Antigen-like Family Member L, SELL, LYAM1, LNHR) (PE)

Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
CD62L; Polyclonal Antibody; CT (L-selectin; Lymph Node Homing Receptor; Leukocyte Adhesion Molecule 1; LAM-1; Leukocyte Surface Antigen Leu-8; TQ1; gp90-MEL; Leukocyte-endothelial Cell Adhesion Molecule 1; LECAM1; CD62 Antigen-like Family Member L; SELL; LYAM1; LNHR) (PE); anti-CD62L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SELL.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Sequence Length
372
Applicable Applications for anti-CD62L antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human SELL (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SELL Antibody (C-term) in Jurkat cell line lysates (35ug/lane).SELL (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of SELL Antibody (C-term) in Jurkat cell line lysates (35ug/lane).SELL (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lymph tissue with SELL Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lymph tissue with SELL Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of jurkat cells using SELL Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of jurkat cells using SELL Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-CD62L antibody
References
1. Zebrowska, A., et.al., Pol J Pathol 60(1), 26-34 (2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42.187
NCBI Official Full Name
selectin L
UniProt Protein Name
L-selectin
UniProt Gene Name
SELL
UniProt Synonym Gene Names
LNHR; LYAM1; LAM-1; LECAM1
UniProt Entry Name
LYAM1_HUMAN

Uniprot Description

SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23-q25

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; protease binding; cell adhesion molecule binding; carbohydrate binding; glycosphingolipid binding

Biological Process: regulation of immune response; cell adhesion; blood coagulation; leukocyte migration; response to ATP

Disease: Iga Nephropathy, Susceptibility To, 1

Similar Products

Product Notes

The CD62L sell (Catalog #AAA6468125) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD62L, CT (L-selectin, Lymph Node Homing Receptor, Leukocyte Adhesion Molecule 1, LAM-1, Leukocyte Surface Antigen Leu-8, TQ1, gp90-MEL, Leukocyte-endothelial Cell Adhesion Molecule 1, LECAM1, CD62 Antigen-like Family Member L, SELL, LYAM1, LNHR) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD62L can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD62L sell for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD62L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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