Rabbit anti-Human, Mouse CD61 Polyclonal Antibody | anti-CD61 antibody

CD61 polyclonal antibody

Cat. Num. AAA3014696

• Gene Names: ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: CD61; Polyclonal Antibody; CD61 polyclonal antibody; Integrin beta-3; Platelet membrane glycoprotein IIIa; Short name=GPIIIa; CD_antigen=CD61; ITGB3; GP3A; GPIIIa; anti-CD61 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CD61 polyclonal antibody detects endogenous levels of CD61 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD61 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human CD61
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of CD61 polyclonal antibody at 1:500 dilutionLaneA: The Kdney, tissue lysate of RatLaneB: The Testis tissue lysate of Rat)

Related Product Information for anti-CD61 antibody

Integrins are heterodimers composed of noncovalently associated transmembrane alpha and beta subunits. The 16alpha and 8beta subunits heterodimerize to produce more than 20 different receptors. Most Integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain Integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster Integrins by binding to adjacent Integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of Integrinmediated responses.

NCBI and Uniprot Product Information

• NCBI GI #: 47078292
• NCBI GeneID: 3690
• NCBI Accession #: NP_000203.2
• NCBI GenBank Nucleotide #: NM_000212.2
• UniProt Accession #: P05106; O15495; Q12806; Q13413; Q14648; Q16499; A0PJW2; D3DXJ8
• Molecular Weight: 87,058 Da
• NCBI Official Full Name: integrin beta-3
• NCBI Official Synonym Full Names: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• NCBI Official Symbol: ITGB3
• NCBI Official Synonym Symbols: GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
• NCBI Protein Information: integrin beta-3; platelet membrane glycoprotein IIIa
• UniProt Protein Name: Integrin beta-3
• UniProt Gene Name: ITGB3
• UniProt Synonym Gene Names: GP3A; GPIIIa
• UniProt Entry Name: ITB3_HUMAN

NCBI Description

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: filopodium membrane; platelet alpha granule membrane; focal adhesion; cell surface; microvillus membrane; integral to plasma membrane; plasma membrane; melanosome; integrin complex; nucleus; receptor complex

Molecular Function: identical protein binding; protein binding; protease binding; extracellular matrix binding; vascular endothelial growth factor receptor 2 binding; fibronectin binding; platelet-derived growth factor receptor binding; protein disulfide isomerase activity; cell adhesion molecule binding; receptor activity

Biological Process: negative regulation of lipid transport; axon guidance; entry of virus into host cell; extracellular matrix organization and biogenesis; protein folding; wound healing; cell-matrix adhesion; smooth muscle cell migration; mesodermal cell differentiation; negative chemotaxis; positive regulation of vascular endothelial growth factor receptor signaling pathway; platelet degranulation; cell-substrate junction assembly; cell growth; cell adhesion; integrin-mediated signaling pathway; platelet activation; regulation of bone resorption; cell migration; negative regulation of lipoprotein metabolic process; angiogenesis involved in wound healing; cell-substrate adhesion; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; activation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; tube development; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Myocardial Infarction, Susceptibility To; Glanzmann Thrombasthenia; Bleeding Disorder, Platelet-type, 16