Rabbit anti-Human, Mouse CD54 Polyclonal Antibody | anti-CD54 antibody

CD54 (Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, ICAM1) (PE)

Cat. Num. AAA6373146

• Gene Names: ICAM1; BB2; CD54; P3.58
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD54; Polyclonal Antibody; CD54 (Intercellular Adhesion Molecule 1; ICAM-1; Major Group Rhinovirus Receptor; ICAM1) (PE); anti-CD54 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ICAM1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CD54 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ICAM1, aa1-532 (NP_000192.1).
• Immunogen Sequence: MAPSSPRPALPALLVLLGALFPGPGNAQTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTREVTVNVLSPRYEIVIITVVAAAVIMGTAGLSTYLYNRQRKIKKYRLQQAQKGTPMKPNTQATPP
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ICAM1 rabbit polyclonal antibody. Western Blot analysis of ICAM1 expression in mouse liver.)

Western Blot (WB)

(ICAM1 rabbit polyclonal antibody. Western Blot analysis of ICAM1 expression in Hela NE.)

Western Blot (WB)

(Western Blot analysis of ICAM1 expression in transfected 293T cell line by ICAM1 polyclonal antibody. Lane 1: ICAM1 transfected lysate (57.8kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between ICAM1 and FGG. HeLa cells were stained with ICAM1 rabbit purified polyclonal 1:1200 and FGG mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Related Product Information for anti-CD54 antibody

CD54 (ICAM-1) is a 85-110kD type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Product Categories/Family for anti-CD54 antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4557878
• NCBI GeneID: 3383
• NCBI Accession #: NP_000192.1
• NCBI GenBank Nucleotide #: NM_000201
• Molecular Weight: 76.5kDa (692aa) 70-100KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: intercellular adhesion molecule 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 1
• NCBI Official Symbol: ICAM1
• NCBI Official Synonym Symbols: BB2; CD54; P3.58
• NCBI Protein Information: intercellular adhesion molecule 1

NCBI Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]