Rabbit anti-Human, Mouse CD3Z Polyclonal Antibody | anti-CD3Z antibody

CD3Z, CT (CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247) (Azide free) (HRP)

Cat. Num. AAA6283218

• Gene Names: CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CD3Z; Polyclonal Antibody; CT (CD247; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD247) (Azide free) (HRP); CD247; anti-CD3Z antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD3Z. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CD3Z antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 113-141 in the C-terminal region of human CD3Z, UniProt Accession #P20963. Species sequence homology: porcine
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CD3Z (arrow) in 293 cell lysates (2ug/lane) using MBS647375 either nontransfected (Lane 1) or transiently transfected with the CD3Z gene (Lane 2).)

Testing Data

(All lanes: MBS647375 (1:4000-1:8000) Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: mouse thymus lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 19kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-CD3Z antibody

T-cell receptor zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma,-delta and-epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response.

Product Categories/Family for anti-CD3Z antibody

Antibodies; CD Markers

References

1. Miyagawa, H., Rheumatology (Oxford) 47 (2), 158-164 (2008). 2. Gorman, C.L., J. Immunol. 180 (2), 1060-1070 (2008). 3. Eleftheriadis, T., Am. J. Nephrol. 28 (1), 152-157 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4557431
• NCBI GeneID: 919
• NCBI Accession #: NP_000725.1
• NCBI GenBank Nucleotide #: NM_000734.3
• UniProt Accession #: P20963; Q5VX13; Q8TAX4; B1AK49; A46461
• Molecular Weight: 18,696 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 zeta chain isoform 2
• NCBI Official Synonym Full Names: CD247 molecule
• NCBI Official Symbol: CD247
• NCBI Official Synonym Symbols: T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
• NCBI Protein Information: T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3
• UniProt Protein Name: T-cell surface glycoprotein CD3 zeta chain
• UniProt Gene Name: CD247
• UniProt Synonym Gene Names: CD3Z; T3Z; TCRZ
• UniProt Entry Name: CD3Z_HUMAN

NCBI Description

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3Z: a T cell surface glycoprotein that is a component of the T cell antigen receptor. Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD3-epsilon results in impaired immune response. Two alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; transmembrane receptor activity

Biological Process: regulation of immune response; viral reproduction; T cell costimulation; innate immune response; T cell receptor signaling pathway; regulation of defense response to virus by virus

Disease: Immunodeficiency 25