Rabbit anti-Human CD3EAP Polyclonal Antibody | anti-CD3EAP antibody

CD3EAP, NT (CD3EAP, ASE1, CAST, PAF49, DNA-directed RNA polymerase I subunit RPA34, A34.5, Antisense to ERCC-1 protein, CD3-epsilon-associated protein, RNA polymerase I-associated factor PAF49) (Biotin)

Cat. Num. AAA6283172

• Gene Names: CD3EAP; ASE1; CAST; ASE-1; PAF49
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD3EAP; Polyclonal Antibody; NT (CD3EAP; ASE1; CAST; PAF49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; CD3-epsilon-associated protein; RNA polymerase I-associated factor PAF49) (Biotin); RNA polymerase I-associated factor PAF49; anti-CD3EAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CD3EAP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CD3EAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-81 amino acids from the N-terminal region of human CD3EAP.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CD3EAP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD3EAP antibody detected the CD3EAP protein (arrow).)

Related Product Information for anti-CD3EAP antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.

Product Categories/Family for anti-CD3EAP antibody

Antibodies; DNA Replication; Repair

References

Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). Vangsted, A.J., et al. Haematologica 94(9):1274-1281(2009). Nissen, K.K., et al. Anticancer Drugs 20(3):174-178(2009). Schierup, M.H., et al. BMC Med. Genet. 10, 20 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6912246
• NCBI GeneID: 10849
• NCBI Accession #: NP_036231.1
• NCBI GenBank Nucleotide #: NM_012099.1
• UniProt Accession #: O15446; Q32N11; Q7Z5U2; Q9UPF6
• Molecular Weight: 54,986 Da
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA34
• NCBI Official Synonym Full Names: CD3e molecule, epsilon associated protein
• NCBI Official Symbol: CD3EAP
• NCBI Official Synonym Symbols: ASE1; CAST; ASE-1; PAF49
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA34; A34.5; antisense to ERCC 1; CD3E-associated protein; antisense to ERCC-1 protein; CD3 epsilon associated protein; CD3-epsilon-associated protein; RNA polymerase I-associated factor PAF49; CD3e antigen, epsilon
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA34
• UniProt Gene Name: CD3EAP
• UniProt Synonym Gene Names: ASE1; CAST; PAF49; ASE-1; CAST
• UniProt Entry Name: RPA34_HUMAN

Uniprot Description

ASE-1: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Belongs to the eukaryotic RPA34 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Transcription initiation complex

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; RNA polymerase I transcription factor complex; chromosome; nucleus; DNA-directed RNA polymerase I complex

Molecular Function: DNA-directed RNA polymerase activity

Biological Process: negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transmembrane receptor protein tyrosine kinase signaling pathway; rRNA transcription