Mouse anti-Human CD33  Monoclonal Antibody | anti-CD33  antibody

CD33  Monoclonal Antibody

Cat. Num. AAA668002

• Gene Names: CD33; p67; SIGLEC3; SIGLEC-3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: CD33 ; Monoclonal Antibody; CD33 Monoclonal Antibody; anti-CD33 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, Kappa
• Clone Number: ABM29D3
• Form/Format: Purified (Liquid)
• Concentration: 0.5 mg/ml, 200 µl (varies by lot)
• Sequence Length: 237

• Applicable Applications for anti-CD33 antibody: Western Blot (WB), Immunohistochemistry (IHC), Fluorescence-activated cell sorting (FACS)
• Application Notes: Western blot analysis: 2-4 ug/ml; Immunohistochemical analysis: 5 ug/ml; FACS: 0.2-0.5 ug/10⁶ cells
• Antigen: CD33
• Immunogen: A partial recombinant CD33 protein (amino acids 28-320) was used as the immunogen for this antibody.
• Preparation and Storage: Store antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles. Expire 1 year from date of purchase.

Western Blot (WB)

(Expression analysis of CD33. Anti- CD33 antibody (Clone:ABM29D3) was used at 2 µg/ml on Human Spleen and DU145 lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of CD33  in adenocarcinoma of rectum using CD33 antibody (Clone: ABM29D3) at 5 ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of CD33  in Renal Cell Carcinoma using CD33 antibody (Clone: ABM29D3) at 5 ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of CD33  in squamous cell carcinoma of esophagus using CD33 antibody (Clone: ABM29D3) at 5 ug/ml.)

Flow Cytometry

(Cell Surface flow analysis of hCD33 in PBMC (Monocytes) using 0.2?g/10^6 cells of CD33 clone (ABM29D3). Green represents isotype control; red represents antihCD33 antibody. Goat anti-mouse PE conjugated secondary antibody was used.)

Related Product Information for anti-CD33  antibody

CD33 is a member of the SIGLEC (Sialic Acid-Binding Ig-Like Lectin) family of receptors, and the gene comprises seven coding exons. Exon 2 encodes the canonical IgV domain, exon 4 encodes the IgC structural domain, and exons 6 and 7 encode cytosolic ITIMs (Immunotyrosine Inhibitory Motifs). CD33 acts as a cell surface antigen which is expressed on normal myeloid cells and CD34+ blasts in AML (Acute Myeloid Leukemia). The antigen serves as a target of GO (Gemtuzumab/Ozogamicin), which exerts anti-leukemic effects in refractory AML.

Product Categories/Family for anti-CD33  antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 130980072
• NCBI GeneID: 945
• NCBI Accession #: NP_001076087.1
• NCBI GenBank Nucleotide #: NM_001082618.1
• UniProt Accession #: P20138; Q8TD24; B4E3P8; C9JEN7; F8WAL2
• NCBI Official Full Name: myeloid cell surface antigen CD33 isoform 2
• NCBI Official Synonym Full Names: CD33 molecule
• NCBI Official Symbol: CD33
• NCBI Official Synonym Symbols: p67; SIGLEC3; SIGLEC-3
• NCBI Protein Information: myeloid cell surface antigen CD33; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3
• UniProt Protein Name: Myeloid cell surface antigen CD33
• UniProt Gene Name: CD33
• UniProt Synonym Gene Names: SIGLEC3; Siglec-3
• UniProt Entry Name: CD33_HUMAN

Uniprot Description

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity; carbohydrate binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion