Rabbit anti-Cynomolgus CD33 Polyclonal Antibody | anti-CD33 antibody
Anti-CD33 Antibody, Rabbit Polyclonal
Reactivity
Cynomolgus
Applications
ELISA
Purity
Protein A
Synonyms
CD33; Polyclonal Antibody; Anti-CD33 Antibody; Rabbit Polyclonal; CD33/Siglec-3 Antibody; Rabbit PAb; CD33 molecule; anti-CD33 antibody
Host
Rabbit
Reactivity
Cynomolgus
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Cynomolgus CD33/Siglec-3
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD33 antibody
ELISA (EIA)
Application Notes
ELISA: 1:1000-1:2000
This antibody can be used at 1:1000-1:2000 with the appropriate secondary reagents to detect Cynomolgus CD33 / Siglec-3.
Optimal concentrations/dilutions should be determined by the end user.
This antibody can be used at 1:1000-1:2000 with the appropriate secondary reagents to detect Cynomolgus CD33 / Siglec-3.
Optimal concentrations/dilutions should be determined by the end user.
Immunogen
Recombinant Cynomolgus CD33/Siglec-3 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus CD33/Siglec-3 (CAT No: MBS8117981) . Total IgG was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Related Product Information for anti-CD33 antibody
Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.
Product Categories/Family for anti-CD33 antibody
References
Simmons D, et al. (1988) Isolation of a cDNA encoding CD33, a differentiation antigen of myeloid progenitor cells. J Immunol. 141(8): 2797-800.
Ulyanova T, et al. (1999) The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 29(11): 3440-9.
Freeman SD, et al. (1995) Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 85(8): 2005-12.
Ulyanova T, et al. (1999) The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 29(11): 3440-9.
Freeman SD, et al. (1995) Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 85(8): 2005-12.
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The CD33 (Catalog #AAA8113301) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD33 Antibody, Rabbit Polyclonal reacts with Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's CD33 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1:1000-1:2000 This antibody can be used at 1:1000-1:2000 with the appropriate secondary reagents to detect Cynomolgus CD33 / Siglec-3. Optimal concentrations/dilutions should be determined by the end user. Researchers should empirically determine the suitability of the CD33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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