Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD33 expression on human whole blood monocytes (left panel) and granulocytes (right panel). Cells were stained with PerCP conjugated anti-Human CD33. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes and granulocytes.)
Rabbit anti-Human CD33 Monoclonal Antibody | anti-CD33 antibody
Recombinant Anti-CD33 Antibody (PerCP), Rabbit Monoclonal
Gene Names
CD33; p67; SIGLEC3; SIGLEC-3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD33; Monoclonal Antibody; Recombinant Anti-CD33 Antibody (PerCP); Rabbit Monoclonal; CD33/Siglec-3 Antibody (PerCP); Rabbit MAb; CD33 molecule; Anti-p67 Antibody; Anti-Siglec-3 Antibody; Anti-SIGLEC3 Antibody; anti-CD33 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
001
Specificity
Human CD33/Siglec-3
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD33 antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human CD33/Siglec-3 Protein
Conjugation
PerCP
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD33/Siglec-3 (rh CD33/Siglec-3) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD33 expression on human whole blood monocytes (left panel) and granulocytes (right panel). Cells were stained with PerCP conjugated anti-Human CD33. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes and granulocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD33 expression on human whole blood monocytes (left panel) and granulocytes (right panel). Cells were stained with PerCP conjugated anti-Human CD33. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes and granulocytes.)
Related Product Information for anti-CD33 antibody
Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.
Product Categories/Family for anti-CD33 antibody
References
Simmons D, et al. (1988) Isolation of a cDNA encoding CD33, a differentiation antigen of myeloid progenitor cells. J Immunol. 141(8): 2797-800.
Ulyanova T, et al. (1999) The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 29(11): 3440-9.
Freeman SD, et al. (1995) Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 85(8): 2005-12.
Ulyanova T, et al. (1999) The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 29(11): 3440-9.
Freeman SD, et al. (1995) Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 85(8): 2005-12.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,293 Da
NCBI Official Full Name
CD33 molecule
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
NCBI Protein Information
myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3
Protein Family
Research Articles on CD33
Similar Products
Product Notes
The CD33 (Catalog #AAA8106309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD33 Antibody (PerCP), Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD33 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the CD33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD33, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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