Rabbit anti-Human CD320 Polyclonal Antibody | anti-CD320 antibody

CD320, CT (CD320, 8D6A, CD320 antigen, 8D6 antigen, FDC-signaling molecule 8D6, Transcobalamin receptor, CD320) (APC)

Cat. Num. AAA6283083

• Gene Names: CD320; 8D6; 8D6A; TCBLR
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CD320; Polyclonal Antibody; CT (CD320; 8D6A; CD320 antigen; 8D6 antigen; FDC-signaling molecule 8D6; Transcobalamin receptor; CD320) (APC); anti-CD320 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD320.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CD320 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 153-182 in the C-terminal region of human CD320, UniProt Accession #Q9NPF0.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in U251 cell line lysates (35ug/lane) usingMBS645008. This demonstrates that MBS645008 detected the CD320 protein (arrow).)

Related Product Information for anti-CD320 antibody

The CD320 antigen gene encodes the transcobalamin receptor (TCBLR). Cellular uptake of cobalamin (vitamin B12) is mediated by receptors expressed on the cell surface. Transcobalamin II (TCN2; MIM 613441), a plasma protein secreted by endothelial cells, binds the cobalamin absorbed in the distal ileum and carries 10 to 30% of total circulating cobalamin. CD320 encodes a transcobalamin receptor that binds TCN2-cobalamin at the plasma membrane and internalizes the complex by endocytosis (Quadros et al., 2009 [PubMed 18779389]).

Product Categories/Family for anti-CD320 antibody

Antibodies; CD Markers

References

1. Jiang, W., et al. Gene 466 (1-2), 49-55 (2010). 2. Pangilinan, F., et al. J. Med. Genet. 47(10):677-685(2010). 3. Quadros, E.V., et al. Hum. Mutat. 31(8):924-929(2010). 4. Quadros, E.V., et al. Blood 113(1):186-192(2009). 5. Cho, W., et al. BMB Rep 41(12):863-867(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 259906403
• NCBI GeneID: 51293
• NCBI Accession #: NP_001159367.1; NP_057663.1
• NCBI GenBank Nucleotide #: NM_001165895.1
• UniProt Accession #: Q9NPF0; Q53HF7; B2RDS5; D6W668; F5H6D3
• Molecular Weight: 24,371 Da
• NCBI Official Full Name: CD320 antigen isoform 2
• NCBI Official Synonym Full Names: CD320 molecule
• NCBI Official Symbol: CD320
• NCBI Official Synonym Symbols: 8D6; 8D6A; TCBLR
• NCBI Protein Information: CD320 antigen; FDC-SM-8D6; 8D6 antigen; transcobalamin receptor; FDC-signaling molecule 8D6
• UniProt Protein Name: CD320 antigen
• Protein Family: CD320 antigen
• UniProt Gene Name: CD320
• UniProt Synonym Gene Names: 8D6A; TCblR

Uniprot Description

Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed:18779389). Plays an important role in cobalamin uptake (PubMed:18779389, PubMed:20524213). Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells (PubMed:10727470). Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation (PubMed:10727470, PubMed:11418631). Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) (PubMed:11418631). CD320 augments the proliferation of PC precursors generated by IL-10 (PubMed:11418631).