Rabbit anti-Human CD31 Polyclonal Antibody | anti-PECAM1 antibody

Anti-CD31 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101300

• Gene Names: PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CD31; Polyclonal Antibody; Anti-CD31 Antibody; Rabbit Polyclonal; CD31/PECAM-1 Antibody; Rabbit PAb; Antigen Affinity Purified; platelet/endothelial cell adhesion molecule 1; Anti-CD31/EndoCAM Antibody; Anti-endoCAM Antibody; Anti-GPIIA' Antibody; Anti-PECA1 Antibody; Anti-PECAM-1 Antibody; anti-PECAM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CD31/PECAM-1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PECAM1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 5-10uL/mg of lysate
• Immunogen: Recombinant Human CD31/PECAM-1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD31/PECAM-1 (rh CD31/PECAM-1). CD31/PECAM-1 specific IgG was purified by Human CD31/PECAM-1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CD31 rabbit polyclonal antibody at 1:1000 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary HRP Conjugated Goat anti-Rabbit IgG (H+L),at 1:10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:83 kDa Observed band size:130 kDa (We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining of human CD31 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human CD31 in human stomach with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of CD31-His in HUVEC cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CD31-His polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cell membrane.)

Immunoprecipitation (IP)

(CD31 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-CD31 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CD31 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa Observed band size :120 kDa)

Related Product Information for anti-PECAM1 antibody

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin alphavbeta3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

Product Categories/Family for anti-PECAM1 antibody

Hemangioblast Markers ITIM/ITAM Immunoreceptors and Related Molecules

References

Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31-human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.

NCBI and Uniprot Product Information

• NCBI GI #: 119614614
• NCBI GeneID: 5175
• Molecular Weight: 81,378 Da
• NCBI Official Full Name: platelet/endothelial cell adhesion molecule (CD31 antigen), isoform CRA_a
• NCBI Official Synonym Full Names: platelet and endothelial cell adhesion molecule 1
• NCBI Official Symbol: PECAM1
• NCBI Official Synonym Symbols: CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• NCBI Protein Information: platelet endothelial cell adhesion molecule
• Protein Family: Platelet endothelial cell adhesion molecule

NCBI Description

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]