Rabbit anti-Human CD2BP2 Polyclonal Antibody | anti-CD2BP2 antibody

CD2BP2, NT (CD2BP2, KIAA1178, CD2 antigen cytoplasmic tail-binding protein 2) (MaxLight 650)

Cat. Num. AAA6283024

• Gene Names: CD2BP2; LIN1; Snu40; FWP010; U5-52K; PPP1R59
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CD2BP2; Polyclonal Antibody; NT (CD2BP2; KIAA1178; CD2 antigen cytoplasmic tail-binding protein 2) (MaxLight 650); CD2 antigen cytoplasmic tail-binding protein 2; anti-CD2BP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD2BP2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-CD2BP2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 24-52 in the N-terminal region of human CD2BP2. Species sequence homology: mouse
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS648731. This demonstrates that MBS648731 detected the CD2BP2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS648731 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS648731 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using MBS648731 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CD2BP2 antibody

Antibodies; CD Markers

References

1. Heinze, M., et al. Int. Immunol. 19(11):1313-1318(2007). 2. Nielsen, T.K., et al. J. Mol. Biol. 369(4):902-908(2007). 3. Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). 4. Olsen, J.V., et al. Cell 127(3):635-648(2006). 5. Al-Dhaheri, M.H., et al. Steroids 71 (11-12), 966-978 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5174409
• NCBI GeneID: 10421
• NCBI Accession #: NP_006101.1
• NCBI GenBank Nucleotide #: NM_006110.2
• UniProt Accession #: O95400; Q9ULP2; B2RDX2
• Molecular Weight: 37,646 Da
• NCBI Official Full Name: CD2 antigen cytoplasmic tail-binding protein 2
• NCBI Official Synonym Full Names: CD2 (cytoplasmic tail) binding protein 2
• NCBI Official Symbol: CD2BP2
• NCBI Official Synonym Symbols: LIN1; Snu40; FWP010; U5-52K; PPP1R59
• NCBI Protein Information: CD2 antigen cytoplasmic tail-binding protein 2
• UniProt Protein Name: CD2 antigen cytoplasmic tail-binding protein 2
• Protein Family: CD2 antigen cytoplasmic tail-binding protein
• UniProt Gene Name: CD2BP2
• UniProt Synonym Gene Names: KIAA1178; CD2 cytoplasmic domain-binding protein 2; CD2 tail-binding protein 2; U5-52K
• UniProt Entry Name: CD2B2_HUMAN

NCBI Description

This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined. [provided by RefSeq, Nov 2008]

Uniprot Description

CD2BP2: Binds the cytoplasmic domain of CD2 through the GYF domain.

Protein type: RNA processing; Spliceosome; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; U4/U6 x U5 tri-snRNP complex; snRNP U5; cytoplasm; nuclear speck; nucleus

Molecular Function: ribonucleoprotein binding; protein binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression