Rabbit CD253 Polyclonal Antibody | anti-CD253 antibody

RABBIT ANTI HUMAN CD253 (C-TERMINAL)

Cat. Num. AAA221579

• Gene Names: TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
• Applications: Immunohistochemistry, Western Blot
• Synonyms: CD253; Polyclonal Antibody; RABBIT ANTI HUMAN CD253 (C-TERMINAL); anti-CD253 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises the 32kD apoptosis related protein designated TRAIL (TNF-related apoptosis-inducing ligand) TRAIL also known as CD253, is a type II membrane protein expressed in a variety of tissues. Receptors for TRAIL have recently been identified and termed DR4 and DR5.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 101

• Applicable Applications for anti-CD253 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; ; Western Blotting: Maximum Dilution: 1/500
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: Peptide from C-terminus of human CD253 (TRAIL); Buffer Solution
• Antiserum Preparation: Antisera to CD253 (TRAIL) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Immunohistochemical staining of human brain with Rabbit anti Human CD253 (MBS221579))

Testing Data #2

(Western blot analysis of HeLa cell lysate transfected with decreasing amounts (10 - 1ng) of recombinant protein containing the extracellular domain of human TRAIL, probed with Rabbit anti Human TRAIL (MBS221579))

Related Product Information for anti-CD253 antibody

MBS221579 recognises the 32kD apoptosis related protein designated TRAIL (TNF-related apoptosis-inducing ligand) TRAIL also known as CD253, is a type II membrane protein expressed in a variety of tissues. Receptors for TRAIL have recently been identified and termed DR4 and DR5.

NCBI and Uniprot Product Information

• NCBI GI #: 300193032
• NCBI GeneID: 8743
• NCBI Accession #: NP_001177871.1
• NCBI GenBank Nucleotide #: NM_001190942.1
• UniProt Accession #: P50591; A1Y9B3
• Molecular Weight: 11,746 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10 isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 10
• NCBI Official Symbol: TNFSF10
• NCBI Official Synonym Symbols: TL2; APO2L; CD253; TRAIL; Apo-2L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• UniProt Gene Name: TNFSF10
• UniProt Synonym Gene Names: APO2L; TRAIL; Apo-2L; Protein TRAIL
• UniProt Entry Name: TNF10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: extracellular space; integral to plasma membrane; extracellular region

Molecular Function: protein binding; metal ion binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; cell-cell signaling; apoptosis; positive regulation of apoptosis; male gonad development; positive regulation of caspase activity; immune response; signal transduction; response to insulin stimulus