PDLIM1 recombinant protein

PDLIM1, 1-329aa, Human, His tag, E Coli

Cat. Num. AAA205695

• Gene Names: PDLIM1; CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: PDLIM1; 1-329aa; Human; His tag; E Coli; PDZ and LIM domain protein 1; CLIM1; CLP-36; CLP36; Hclim1; PDLIM1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT, 2mM EDTA
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMTTQQ IDLQGPGPWG FRLVGGKDFE QPLAISRVTP GSKAALANLC IGDVITAIDG ENTSNMTHLE AQNRIKGCTD NLTLTVARSE HKVWSPLVTE EGKRHPYKMN LASEPQEVLH IGSAHNRSAM PFTASPASST TARVITNQYN NPAGLYSSEN ISNFNNALES KTAASGVEAN SRPLDHAQPP SSLVIDKESE VYKMLQEKQE LNEPPKQSTS FLVLQEILES EEKGDPNKPS GFRSVKAPVT KVAASIGNAQ KLPMCDKCGT GIVGVFVKLR DRHRHPECYV CTDCGTNLKQ KGHFFVEDQI YCEKHARERV TPPEGYEVVT VFPK
• Sequence Length: 329

• Applicable Applications for PDLIM1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PDLIM1 recombinant protein

PDLIM1 is a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. Recombinant human PDLIM1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PDLIM1 recombinant protein

Metabolism

References

Bauer K., et al. (2000) Blood. 96: 4236-4245.; Vallenius T., et al. (2002) J Cell Sci. 115: 2067-2073.;

NCBI and Uniprot Product Information

• NCBI GI #: 13994151
• NCBI GeneID: 9124
• NCBI Accession #: NP_066272
• NCBI GenBank Nucleotide #: NM_020992.3
• UniProt Accession #: O00151; Q5VZH5; Q9BPZ9; B2RBS6
• Molecular Weight: 38.7 kDa (354aa) confirmed by MALDI-TOF
• NCBI Official Full Name: PDZ and LIM domain protein 1
• NCBI Official Synonym Full Names: PDZ and LIM domain 1
• NCBI Official Symbol: PDLIM1
• NCBI Official Synonym Symbols: CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112
• NCBI Protein Information: PDZ and LIM domain protein 1
• UniProt Protein Name: PDZ and LIM domain protein 1
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM1
• UniProt Synonym Gene Names: CLIM1; CLP36

NCBI Description

This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012]

Uniprot Description

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed:10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts ().