Chemiluminescence
(MBS625012(2µg/ml) staining of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas G Clarkson, Sir William Dunn School of Pathology, Oxford, UK)
Goat anti-Human CD244 Polyclonal Antibody | anti-CD244 antibody
CD244 (CD244 Molecule, Natural Killer Cell Receptor, 2B4)
Gene Names
CD244; 2B4; NAIL; Nmrk; NKR2B4; SLAMF4
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by peptide affinity chromatography.
Synonyms
CD244; Polyclonal Antibody; CD244 (CD244 Molecule; Natural Killer Cell Receptor; 2B4); Anti -CD244 (CD244 Molecule; anti-CD244 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CD244.
Purity/Purification
Affinity Purified
Purified by peptide affinity chromatography.
Purified by peptide affinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Applicable Applications for anti-CD244 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Peptide ELISA Titer: 1:64,000
Western Blot: 1-3ug/ml ~75kD band observed in Human Natural Killer (NK) cell lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1kD according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected.
Dilution: Peptide ELISA Titer: 1:64,000
Western Blot: 1-3ug/ml ~75kD band observed in Human Natural Killer (NK) cell lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1kD according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected.
Immunogen
Synthetic peptide corresponding to C-EFLTIpYEDVKD, from the internal region of human CD244 (NP_057466.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
(MBS625012(2µg/ml) staining of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas G Clarkson, Sir William Dunn School of Pathology, Oxford, UK)
Chemiluminescence
(MBS625012(2µg/ml) staining of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas G Clarkson, Sir William Dunn School of Pathology, Oxford, UK)
Related Product Information for anti-CD244 antibody
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-CD244 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,616 Da
NCBI Official Full Name
CD244 molecule, natural killer cell receptor 2B4
NCBI Official Synonym Full Names
CD244 molecule, natural killer cell receptor 2B4
NCBI Official Symbol
CD244
NCBI Official Synonym Symbols
2B4; NAIL; Nmrk; NKR2B4; SLAMF4
NCBI Protein Information
natural killer cell receptor 2B4; h2B4; OTTHUMP00000027884; OTTHUMP00000027885; OTTHUMP00000027886; OTTHUMP00000027887; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NK cell activation inducing ligand NAIL
UniProt Protein Name
Natural killer cell receptor 2B4
Protein Family
UniProt Gene Name
CD244
UniProt Synonym Gene Names
2B4
UniProt Entry Name
CD244_HUMAN
NCBI Description
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Uniprot Description
CD244: Modulate other receptor-ligand interactions to enhance leukocyte activation. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; receptor activity
Biological Process: signal transduction; blood coagulation; leukocyte migration
Disease: Rheumatoid Arthritis
Research Articles on CD244
Similar Products
Product Notes
The CD244 cd244 (Catalog #AAA625012) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The CD244 (CD244 Molecule, Natural Killer Cell Receptor, 2B4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD244 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Peptide ELISA Titer: 1:64,000 Western Blot: 1-3ug/ml ~75kD band observed in Human Natural Killer (NK) cell lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1kD according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected. Researchers should empirically determine the suitability of the CD244 cd244 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD244, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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