Rabbit anti-Rhesus CD22 Polyclonal Antibody | anti-CD22 antibody

Anti-CD22 Antibody, Rabbit Polyclonal

Cat. Num. AAA8113273

• Reactivity: Rhesus
• Applications: ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: CD22; Polyclonal Antibody; Anti-CD22 Antibody; Rabbit Polyclonal; CD22 Antibody; Rabbit PAb; Antigen Affinity Purified; CD22 molecule; anti-CD22 antibody

• Host: Rabbit
• Reactivity: Rhesus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rhesus CD22
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-CD22 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Rhesus CD22 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rhesus CD22. CD22 specific IgG was purified by Rhesus CD22 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CD22 antibody

CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2, 6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.

Product Categories/Family for anti-CD22 antibody

Cancer Drug Targets ITIM/ITAM Immunoreceptors and Related Molecules; B Cell Receptor Signaling Pathway

References

Tedder TF, et al. (1997) CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol. 15: 481-504.
Tedder TF, et al. (2005) CD22: a multifunctional receptor that regulates B lymphocyte survival and signal transduction. Adv Immunol. 88: 1-50.
Fujimoto M, et al. (2007) B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J Dermatol Sci. 46(1): 1-9.
Walker JA, et al. (2008) CD22: an inhibitory enigma. Immunology. 123(3): 314-25.
Nitschke L. (2009) CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 230(1): 128-43.

NCBI and Uniprot Product Information

• Protein Family: CD226 antigen