Immunohistochemical
(Immunohistochemical analysis of FFPE Human colon cancer sections expressing CD20. MBS551023 was used as primary antibody, dilution 1:500, DAB chromogenic substrate.)
Rabbit CD20 Polyclonal Antibody | anti-CD20 antibody
Rabbit anti CD20 Polyclonal
WB: A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
Immunohistochemical
(Immunohistochemical analysis of FFPE Human colon cancer sections expressing CD20. MBS551023 was used as primary antibody, dilution 1:500, DAB chromogenic substrate.)
Immunohistochemical
(Immunohistochemical analysis of FFPE Human colon cancer sections expressing CD20. MBS551023 was used as primary antibody, dilution 1:500, DAB chromogenic substrate.)
Immunohistochemical
(Immunohistochemical analysis of FFPE mouse groin lymphoma sections expressing CD20. MBS551023 was used as primary antibody, dilution 1:500, DAB chromogenic substrate.)
Immunohistochemical
(Immunohistochemical analysis of FFPE mouse groin lymphoma sections expressing CD20. MBS551023 was used as primary antibody, dilution 1:500, DAB chromogenic substrate.)
• increasing the efficacy of CD20 antibody therapy through the engineering of a new type ii anti-CD20 antibody with enhanced direct and immune effector cell–mediated b-cell cytotoxicity .Blood, Jun 2010; 115: 4393 - 4402.
• antifungal therapy with itraconazole impairs the anti-lymphoma effects of rituximab by inhibiting recruitment of CD20 to cell surface lipid rafts .Cancer Res., Jun 2010; 70: 4292 - 4296.
• expanded cd23+/cd21hi b cells in inflamed lymph nodes are associated with the onset of inflammatory-erosive arthritis in tnf-transgenic mice and are targets of anti-CD20 therapy .J. Immunol., Jun 2010; 184: 6142 - 6150.
• CD20-immunotargeting of malignant melanoma. .ASCO Meeting Abstracts, May 2010; 28: 8565.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.
Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11q12
Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane
Molecular Function: epidermal growth factor receptor binding
Biological Process: B cell proliferation; humoral immune response
Disease: Immunodeficiency, Common Variable, 5
Similar Products
Product Notes
The CD20 ms4a1 (Catalog #AAA551023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CD20 Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA. ELISA: Use at a concentration range of 1-2ug/ml. WB: A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Researchers should empirically determine the suitability of the CD20 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
