Western Blot (WB)
(Western Blot; Sample: Lane1: Equine Spleen lysate; Lane2: HepG2 cell lysate Primary Ab: 1ug/ml Rabbi)
Rabbit anti-Horse Interleukin 18 (IL18) Polyclonal Antibody | anti-IL18 antibody
Polyclonal Antibody to Interleukin 18 (IL18)
Gene Names
IL18; IGIF; IL-18
Reactivity
Horse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Interleukin 18 (IL18); Polyclonal Antibody; Polyclonal Antibody to Interleukin 18 (IL18); IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma; anti-IL18 antibody
Host
Rabbit
Reactivity
Horse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against IL18. It has been selected for its ability to recognize IL18 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence
Tyr37~Asn193
Applicable Applications for anti-IL18 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:250-2500
IHC: 5-20ug/mL; 1:25-100
ICC: 5-20ug/mL; 1:25-100
IHC: 5-20ug/mL; 1:25-100
ICC: 5-20ug/mL; 1:25-100
Immunogen Sequence
Tyr37~Asn193
Cross Reactivity
Horse
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Lane1: Equine Spleen lysate; Lane2: HepG2 cell lysate Primary Ab: 1ug/ml Rabbi)
Western Blot (WB)
(Western Blot; Sample: Lane1: Equine Spleen lysate; Lane2: HepG2 cell lysate Primary Ab: 1ug/ml Rabbi)
Product Categories/Family for anti-IL18 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,058 Da
NCBI Official Full Name
interleukin-18
NCBI Official Synonym Full Names
interleukin 18
NCBI Official Symbol
IL18
NCBI Official Synonym Symbols
IGIF; IL-18
NCBI Protein Information
interleukin-18
UniProt Protein Name
Interleukin-18
Protein Family
UniProt Gene Name
IL18
UniProt Synonym Gene Names
IGIF; IL-18; IFN-gamma-inducing factor; IL-1 gamma
Uniprot Description
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
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Product Notes
The IL18 il18 (Catalog #AAA2111755) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interleukin 18 (IL18) reacts with Horse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 18 (IL18) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:250-2500 IHC: 5-20ug/mL; 1:25-100 ICC: 5-20ug/mL; 1:25-100. Researchers should empirically determine the suitability of the IL18 il18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Tyr37~Asn1 93. It is sometimes possible for the material contained within the vial of "Interleukin 18 (IL18), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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