Western Blot (WB)
(Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term). MBS9204578 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Rabbit anti-Human CD19 Polyclonal Antibody | anti-CD19 antibody
CD19 Antibody (N-term)
This antibody is purified through a protein A column, followed by peptide affinity purification.
This antibody is purified through a protein A column, followed by peptide affinity purification.
IF~~1:10~50
FC~~1:10~50
IHC-P~~1:10~50
Western Blot (WB)
(Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term). MBS9204578 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term). MBS9204578 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(CD19 Antibody (N-term) MBS9204578 western blot analysis in Raji cell line lysates (35ug/lane).This demonstrates the CD19 antibody detected the CD19 protein (arrow).)
Western Blot (WB)
(CD19 Antibody (N-term) MBS9204578 western blot analysis in Raji cell line lysates (35ug/lane).This demonstrates the CD19 antibody detected the CD19 protein (arrow).)
Western Blot (WB)
(Western blot analysis of CD19 (arrow) using rabbit polyclonal CD19 Antibody (N-term) MBS9204578. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of CD19 (arrow) using rabbit polyclonal CD19 Antibody (N-term) MBS9204578. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2) (Origene Technologies).)
Immunofluorescence (IF)
(Immunofluorescence analysis of CD19 Antibody (N-term) with paraffin-embedded human lymph tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
Immunofluorescence (IF)
(Immunofluorescence analysis of CD19 Antibody (N-term) with paraffin-embedded human lymph tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Bradbury,L.E., J. Immunol. 149 (9), 2841-2850 (1992)
Kozmik,Z., Mol. Cell. Biol. 12 (6), 2662-2672 (1992)
NCBI and Uniprot Product Information
NCBI Description
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
Uniprot Description
CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice.
Protein type: Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: protein complex; integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: receptor signaling protein activity
Biological Process: epidermal growth factor receptor signaling pathway; regulation of immune response; B cell receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cell surface receptor linked signal transduction; nerve growth factor receptor signaling pathway; cellular defense response; innate immune response; positive regulation of release of sequestered calcium ion into cytosol
Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 3
Research Articles on CD19
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Product Notes
The CD19 cd19 (Catalog #AAA9204578) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 143-172. The CD19 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD19 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA). WB~~1:500 IF~~1:10~50 FC~~1:10~50 IHC-P~~1:10~50. Researchers should empirically determine the suitability of the CD19 cd19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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