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Dot Blot (DB) (Dot blot analysis of anti-Phospho-SLAMF1-pY281 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Rabbit anti-Human CD150 Polyclonal Antibody | anti-CD150 antibody

CD150, Phosphorylated (Tyr281) (Signaling Lymphocytic Activation Molecule, IPO-3, CDw150, SLAMF1, SLAM) (AP)

Gene Names
SLAMF1; SLAM; CD150; CDw150
Reactivity
Human
Applications
ELISA, Dot Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CD150; Polyclonal Antibody; Phosphorylated (Tyr281) (Signaling Lymphocytic Activation Molecule; IPO-3; CDw150; SLAMF1; SLAM) (AP); anti-CD150 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SLAMF1 when phosphorylated at Tyr281.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-CD150 antibody
ELISA (EIA), Dot Blot (DB)
Application Notes
ELISA: 1:1,000
Dot Blot: 1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr281 of human SLAMF1 (KLH).
Conjugate
AP
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-SLAMF1-pY281 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Dot Blot (DB) (Dot blot analysis of anti-Phospho-SLAMF1-pY281 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Product Categories/Family for anti-CD150 antibody
References
1. Mehrle, S., Mol. Immunol. 45(3), 796-804 (2008). 2. Howie, D., Blood 99(3), 957-965 (2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,839 Da
NCBI Official Full Name
signaling lymphocytic activation molecule
NCBI Official Synonym Full Names
signaling lymphocytic activation molecule family member 1
NCBI Official Symbol
SLAMF1
NCBI Official Synonym Symbols
SLAM; CD150; CDw150
NCBI Protein Information
signaling lymphocytic activation molecule; IPO-3
UniProt Protein Name
Signaling lymphocytic activation molecule
UniProt Gene Name
SLAMF1
UniProt Synonym Gene Names
SLAM
UniProt Entry Name
SLAF1_HUMAN

Uniprot Description

SLAM: a type I membrane protein present on the surface of B cells, T cells and CD83+ dendritic cells. A high-affinity self-ligand considered to be important in bidirectional T <-> B-cell stimulation. SLAM-induced signal-transduction events in T lymphocytes are different from those in B cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A (SAP) acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (SHP-2)-dependent signal transduction operates. Its cytoplasmic domain interacts with SAP through part of its SH2 domain, and with SHP-2. Three alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to membrane; phagocytic vesicle; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; antigen binding

Biological Process: viral reproduction; regulation of catalytic activity; positive regulation of cell proliferation; regulation of vesicle fusion; lymphocyte activation; signal transduction

Research Articles on CD150

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Product Notes

The CD150 slamf1 (Catalog #AAA6468236) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD150, Phosphorylated (Tyr281) (Signaling Lymphocytic Activation Molecule, IPO-3, CDw150, SLAMF1, SLAM) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD150 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Dot Blot (DB). ELISA: 1:1,000 Dot Blot: 1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD150 slamf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD150, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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