Western Blot (WB)
(Western blot analysis of ANPEP antibody (Center) in SK-Br-3 cell line lysates (35ug/lane). ANPEP (arrow) was detected using the purified Pab.)
Rabbit anti-Human CD13 Polyclonal Antibody
CD13, ID (Aminopeptidase N, AP-N, hAPN, Alanyl Aminopeptidase, Microsomal Aminopeptidase, Aminopeptidase M, AP-M, gp150, Myeloid Plasma Membrane Glycoprotein CD13, ANPEP, PEPN, APN)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
CD13; Polyclonal Antibody; ID (Aminopeptidase N; AP-N; hAPN; Alanyl Aminopeptidase; Microsomal Aminopeptidase; Aminopeptidase M; AP-M; gp150; Myeloid Plasma Membrane Glycoprotein CD13; ANPEP; PEPN; APN); Anti -CD13; anti-CD13 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ANPEP.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-CD13 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunogen
Synthetic peptide selected from the Center region of human ANPEP (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ANPEP antibody (Center) in SK-Br-3 cell line lysates (35ug/lane). ANPEP (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ANPEP antibody (Center) in SK-Br-3 cell line lysates (35ug/lane). ANPEP (arrow) was detected using the purified Pab.)
Related Product Information for anti-CD13 antibody
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.
Product Categories/Family for anti-CD13 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
CD13 antigen
Similar Products
Product Notes
The CD13 (Catalog #AAA629319) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD13, ID (Aminopeptidase N, AP-N, hAPN, Alanyl Aminopeptidase, Microsomal Aminopeptidase, Aminopeptidase M, AP-M, gp150, Myeloid Plasma Membrane Glycoprotein CD13, ANPEP, PEPN, APN) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD13 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the CD13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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