Western Blot (WB)
(Western blot analysis of human CD120b (soluble) using recombinant human CD120b (soluble) diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Human CD120b (soluble) antibody (MBS226103) followed by a compatible secondary antibody.)
Rabbit anti-Human CD120b Polyclonal Antibody | anti-CD120b antibody
Rabbit anti Human CD120b (soluble):Preservative Free
Purified IgG - lyophilized
WB: 0.1ug/ml to 0.2ug/ml
After reconstitution store at -20 degree C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis of human CD120b (soluble) using recombinant human CD120b (soluble) diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Human CD120b (soluble) antibody (MBS226103) followed by a compatible secondary antibody.)
Western Blot (WB)
(Western blot analysis of human CD120b (soluble) using recombinant human CD120b (soluble) diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Human CD120b (soluble) antibody (MBS226103) followed by a compatible secondary antibody.)
CD120b is a member of the TNF receptor superfamily and is a single spanning type I transmembrane receptor. Upon binding of its ligand TNF, the adaptor proteins TRAF-2 and TRAF-2-associated proteins are recruited. This results in the activation of the NF-kappaB signaling pathway as well as the activation of other signaling kinases (Wajant & Siegmund 2019). TNF-CD120b signaling promotes cell proliferation and survival (Ahmad et al. 2018).
A soluble form of CD120b has been described and is produced as a result of alternative splicing or shedding. The soluble form competes with the membrane bound form for the ligand TNF. The binding of TNF to soluble CD120b effectively inhibits TNF signaling by competing with the membrane bound form (Wajant & Siegmund 2019).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]
Uniprot Description
TNF-R2: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Binds to TRAF2. Interacts with BMX. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 1p36.22
Cellular Component: cell soma; perinuclear region of cytoplasm; extracellular region; integral to membrane; plasma membrane; nucleus; lipid raft
Molecular Function: protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding
Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; tumor necrosis factor-mediated signaling pathway; negative regulation of inflammatory response; immune response; RNA destabilization; inflammatory response; positive regulation of membrane protein ectodomain proteolysis; aging
Research Articles on CD120b
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Product Notes
The CD120b tnfrsf1b (Catalog #AAA226103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Human CD120b (soluble):Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD120b can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 0.5ug/ml to 2.0ug/ml WB: 0.1ug/ml to 0.2ug/ml. Researchers should empirically determine the suitability of the CD120b tnfrsf1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD120b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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