Rabbit anti-Human, Mouse CD106 Polyclonal Antibody | anti-CD106 antibody

CD106 (CD106 Antigen, DKFZp779G2333, INCAM 100, INCAM-100, L1CAM, MGC99561, Vascular Cell Adhesion Molecule 1, VCAM 1, VCAM1, VCAM-1, Vascular Cell Adhesion Protein 1) (HRP)

Cat. Num. AAA6372744

• Gene Names: VCAM1; CD106; INCAM-100
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD106; Polyclonal Antibody; CD106 (CD106 Antigen; DKFZp779G2333; INCAM 100; INCAM-100; L1CAM; MGC99561; Vascular Cell Adhesion Molecule 1; VCAM 1; VCAM1; VCAM-1; Vascular Cell Adhesion Protein 1) (HRP); anti-CD106 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VCAM1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CD106 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human VCAM1, aa1-739 (NP_001069.1).
• Immunogen Sequence: MPGKMVVILGASNILWIMFAASQAFKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPELLVLYFASSLIIPAIGMIIYFARKANMKGSYSLVEAQKSKV
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(VCAM1 rabbit polyclonal antibody. Western Blot analysis of VCAM1 expression in mouse lung.)

Western Blot (WB)

(VCAM1 rabbit polyclonal antibody. Western Blot analysis of VCAM1 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of VCAM1 expression in transfected 293T cell line by VCAM1 polyclonal antibody. Lane 1: VCAM1 transfected lysate (81.3kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-CD106 antibody

VCAM-1, which is present on activated endothelial cells. VCAM-1 is an adhesion molecule, which binds to the integrin alpha4/beta1 (VLA-4) and to the integrin alpha4/beta7 on lymphocytes. This antibody has been shown to inhibit T lymphocyte adhesion to TNF stimulated endothelial cells. The molecular weight of the recognized antigen is 110kD.

Product Categories/Family for anti-CD106 antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4507875
• NCBI GeneID: 7412
• NCBI Accession #: NP_001069.1
• NCBI GenBank Nucleotide #: NM_001078
• UniProt Accession #: P19320; Q6NUP8; A8K6R7; B4DKS4; E9PDD1
• Molecular Weight: 739
• NCBI Official Full Name: vascular cell adhesion protein 1 isoform a
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: VCAM1
• NCBI Official Synonym Symbols: CD106; INCAM-100
• NCBI Protein Information: vascular cell adhesion protein 1; CD106 antigen
• UniProt Protein Name: Vascular cell adhesion protein 1
• UniProt Gene Name: VCAM1
• UniProt Synonym Gene Names: L1CAM; V-CAM 1; VCAM-1
• UniProt Entry Name: VCAM1_HUMAN

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: Golgi apparatus; extracellular space; cell surface; microvillus; endoplasmic reticulum; early endosome; integral to membrane; apical part of cell; plasma membrane; podosome; sarcolemma; filopodium; external side of plasma membrane

Molecular Function: amine oxidase activity; integrin binding; cell adhesion molecule binding

Biological Process: response to nicotine; chronic inflammatory response; extracellular matrix organization and biogenesis; regulation of immune response; viral reproduction; cell-matrix adhesion; cytokine and chemokine mediated signaling pathway; heart development; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; B cell differentiation; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; amine metabolic process; response to ionizing radiation; cell adhesion; leukocyte tethering or rolling; response to nutrient; acute inflammatory response; aging