Rabbit anti-Human CCT7 Polyclonal Antibody | anti-CCT7 antibody

CCT7, NT (CCT7, CCTH, NIP7-1, T-complex protein 1 subunit eta, CCT-eta, HIV-1 Nef-interacting protein) (Biotin)

Cat. Num. AAA6282676

• Gene Names: CCT7; CCTH; CCTETA; NIP7-1; TCP1ETA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCT7; Polyclonal Antibody; NT (CCT7; CCTH; NIP7-1; T-complex protein 1 subunit eta; CCT-eta; HIV-1 Nef-interacting protein) (Biotin); HIV-1 Nef-interacting protein; anti-CCT7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CCT7 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CCT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the N-terminal region of human CCT7.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CCT7 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CCT7 antibody detected the CCT7 protein (arrow).)

Related Product Information for anti-CCT7 antibody

This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6.

Product Categories/Family for anti-CCT7 antibody

Antibodies; Infectious Disease HIV

References

Mukherjee, K., et al. BMC Evol. Biol. 10, 64 (2010): Zebol, J.R., et al. Int. J. Biochem. Cell Biol. 41(4):822-827(2009). Guo, D., et al. Biochem. Biophys. Res. Commun. 337(4):1308-1318(2005). Hanafy, K.A., et al. J. Biol. Chem. 279(45):46946-46953(2004). Imai, Y., et al. J. Biol. Chem. 278(51):51901-51910(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 5453607
• NCBI GeneID: 10574
• NCBI Accession #: NP_006420.1
• NCBI GenBank Nucleotide #: NM_006429.3
• UniProt Accession #: Q99832; O14871; Q6FI26; A8K7E6; A8MWI8; B7WNW9; B7Z4T9; B7Z4Z7
• Molecular Weight: 50,352 Da
• NCBI Official Full Name: T-complex protein 1 subunit eta isoform a
• NCBI Official Synonym Full Names: chaperonin containing TCP1, subunit 7 (eta)
• NCBI Official Symbol: CCT7
• NCBI Official Synonym Symbols: CCTH; CCTETA; NIP7-1; TCP1ETA
• NCBI Protein Information: T-complex protein 1 subunit eta; CCT-eta; TCP-1-eta; HIV-1 Nef interacting protein; HIV-1 Nef-interacting protein; chaperonin containing t-complex polypeptide 1, eta subunit
• UniProt Protein Name: T-complex protein 1 subunit eta
• Protein Family: Probable T-complex protein
• UniProt Gene Name: CCT7
• UniProt Synonym Gene Names: CCTH; NIP7-1; TCP-1-eta
• UniProt Entry Name: TCPH_HUMAN

NCBI Description

This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6. [provided by RefSeq, Oct 2009]

Uniprot Description

CCT-eta: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 2p13.2

Cellular Component: chaperonin-containing T-complex; microtubule; mitochondrion; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; unfolded protein binding; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida