Rabbit anti-Human CCT5 Polyclonal Antibody | anti-CCT5 antibody

CCT5 Antibody

Cat. Num. AAA7112828

• Gene Names: CCT5; CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >95%; Protein G Purified
• Synonyms: CCT5; Polyclonal Antibody; CCT5 Antibody; T-complex protein 1 subunit epsilon; TCP-1-epsilon; CCT-epsilon; CCTE; KIAA0098; anti-CCT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%; Protein G Purified
• Form/Format: Liquid
• Sequence Length: 448

• Applicable Applications for anti-CCT5 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IF: 1:50-1:200
• Immunogen Species: Human
• Immunogen: Recombinant Human T-complex protein 1 subunit epsilon protein (1-270AA)
• Conjugate: Non-Conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysateAll lanes: CCT5 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60, 50 kDaObserved band size: 60 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of A549 cells with CSB-PA004862EA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Product Categories/Family for anti-CCT5 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 807066364
• NCBI GeneID: 22948
• NCBI Accession #: NP_001293084.1
• NCBI GenBank Nucleotide #: NM_001306155.1
• UniProt Accession #: P48643; A8JZY8; A8K2X8; B4DYD8
• Molecular Weight: 49,526 Da
• NCBI Official Full Name: T-complex protein 1 subunit epsilon isoform d
• NCBI Official Synonym Full Names: chaperonin containing TCP1 subunit 5
• NCBI Official Symbol: CCT5
• NCBI Official Synonym Symbols: CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
• NCBI Protein Information: T-complex protein 1 subunit epsilon
• UniProt Protein Name: T-complex protein 1 subunit epsilon
• Protein Family: T-complex protein
• UniProt Gene Name: CCT5
• UniProt Synonym Gene Names: TCP-1-epsilon

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015]

Uniprot Description

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).