Rabbit CCT3 Polyclonal Antibody | anti-CCT3 antibody

CCT3 Polyclonal Antibody

Cat. Num. AAA3008237

• Gene Names: CCT3; CCTG; PIG48; TRIC5; CCT-gamma; TCP-1-gamma
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CCT3; Polyclonal Antibody; CCT3 Polyclonal Antibody; T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5; CCTG; TRIC5; anti-CCT3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CCT3 polyclonal antibody detects endogenous levels of CCT3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 507

• Applicable Applications for anti-CCT3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human CCT3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CCT3 polyclonal antibody)

Related Product Information for anti-CCT3 antibody

The protein TCP-1 (t complex polypeptide 1) is a subunit of the hetero-oligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species, TCP-1 alpha, beta, gamma, delta, epsilon, zeta, eta and theta, each encoded by a different gene. Two zeta subunits have been described: TCP-1 zeta (also designated TCP-1 zeta1) and TCP-1 zeta2. TCP-1 subunits are proposed to have independent functions in folding its in vivo substrates, the actins and tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 58761484
• NCBI GeneID: 7203
• NCBI Accession #: NP_001008800.1
• NCBI GenBank Nucleotide #: NP_001008800.1
• UniProt Accession #: P49368
• Molecular Weight: ~ 61kDa
• NCBI Official Full Name: T-complex protein 1 subunit gamma isoform c
• NCBI Official Synonym Full Names: chaperonin containing TCP1 subunit 3
• NCBI Official Symbol: CCT3
• NCBI Official Synonym Symbols: CCTG; PIG48; TRIC5; CCT-gamma; TCP-1-gamma
• NCBI Protein Information: T-complex protein 1 subunit gamma
• UniProt Protein Name: T-complex protein 1 subunit gamma
• Protein Family: Thermosome
• UniProt Gene Name: CCT3
• UniProt Synonym Gene Names: CCTG; TRIC5; TCP-1-gamma
• UniProt Entry Name: TCPG_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq, Aug 2010]

Uniprot Description

CCT3: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Chaperone

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: chaperonin-containing T-complex; cytoplasm; cytoskeleton; cytosol; microtubule; plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of telomere maintenance via telomerase; protein folding; protein stabilization