Human Interleukin 12B (IL12B) RTU ELISA Kit | IL12B rtu elisa kit

Human Interleukin 12B (IL12B) ELISA Kit

Cat. Num. AAA4500196

• Gene Names: IL12B; CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
• Reactivity: Human
• Synonyms: Interleukin 12B (IL12B); Human Interleukin 12B (IL12B) ELISA Kit; p40; IL12-B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23; P40 Subunit; IL12B rtu elisa kit

• Reactivity: Human
• Sequence Length: 328

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 11.84pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL12B rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL12B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL12B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL12B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL12B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 24497438
• NCBI GeneID: 3593
• NCBI Accession #: NP_002178.2
• NCBI GenBank Nucleotide #: NM_002187.2
• UniProt Accession #: P29460
• Molecular Weight: 37,169 Da
• NCBI Official Full Name: interleukin-12 subunit beta
• NCBI Official Synonym Full Names: interleukin 12B
• NCBI Official Symbol: IL12B
• NCBI Official Synonym Symbols: CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
• NCBI Protein Information: interleukin-12 subunit beta
• UniProt Protein Name: Interleukin-12 subunit beta
• Protein Family: Interleukin
• UniProt Gene Name: IL12B
• UniProt Synonym Gene Names: NKSF2; IL-12B; CLMF p40; NKSF2

NCBI Description

This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.