Rabbit anti-Human CCR9 Polyclonal Antibody | anti-CCR9 antibody

CCR9, CT (CCR9, GPR28, C-C chemokine receptor type 9, G-protein coupled receptor 28, GPR-9-6, CDw199) (Azide free) (HRP)

Cat. Num. AAA6282645

• Gene Names: CCR9; GPR28; CDw199; GPR-9-6; CC-CKR-9
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCR9; Polyclonal Antibody; CT (CCR9; GPR28; C-C chemokine receptor type 9; G-protein coupled receptor 28; GPR-9-6; CDw199) (Azide free) (HRP); CDw199; anti-CCR9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CCR9 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CCR9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the C-terminal region of human CCR9.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CCR9 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CCR9 antibody detected the CCR9 protein (arrow).)

Related Product Information for anti-CCR9 antibody

The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.

Product Categories/Family for anti-CCR9 antibody

Antibodies; CD Markers

References

Han, S., et al. Hum. Immunol. 71(7):727-730(2010). Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010). Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). Segat, L., et al. Vaccine 28(10):2201-2206(2010). Wang, Y., et al. Cell. Mol. Immunol. 7(1):51-60(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 14043044
• NCBI GeneID: 10803
• NCBI Accession #: NP_006632.2; NP_112477.1
• NCBI GenBank Nucleotide #: NM_006641.3
• UniProt Accession #: P51686; Q4VBM3; Q549E0; Q9UQQ6
• Molecular Weight: 40,713 Da
• NCBI Official Full Name: C-C chemokine receptor type 9 isoform B
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 9
• NCBI Official Symbol: CCR9
• NCBI Official Synonym Symbols: GPR28; CDw199; GPR-9-6; CC-CKR-9
• NCBI Protein Information: C-C chemokine receptor type 9; G protein-coupled receptor 28
• UniProt Protein Name: C-C chemokine receptor type 9
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR9
• UniProt Synonym Gene Names: GPR28; C-C CKR-9; CC-CKR-9; CCR-9
• UniProt Entry Name: CCR9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

CCR9: Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: C-C chemokine receptor activity; chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cellular defense response; immune response; chemotaxis