Rabbit anti-Human CCR5 Polyclonal Antibody | anti-CCR5 antibody

CCR5 (Phospho-Ser349) Antibody

Cat. Num. AAA9413979

• Gene Names: CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: CCR5; Polyclonal Antibody; CCR5 (Phospho-Ser349) Antibody; C-C CKR-5; CKR5; CHEMR13; CMKBR5; CCR-5; anti-CCR5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of CCR5 only when phosphorylated at serine 349.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 352

• Applicable Applications for anti-CCR5 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 349 (E-I-S(p)-V-G) derived from Human CCR5.
• Modification: Phospho-Ser349
• Target Name: CCR5
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from A549 cells (Lane 2), using CCR5 (Phospho-Ser349) Antibody. The lane on the left is treated with antigen-specific peptide.)

Related Product Information for anti-CCR5 antibody

Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.Martin Oppermann, J. Biol. Chem., Mar 1999; 274: 8875.Beatrix Pollok-Kopp, J. Biol. Chem., Jan 2003; 278: 2190 - 2198.Ramtin Rahbar, J. Virol., Jul 2006; 80: 7245 - 7259.H Olbrich, J. Leukoc. Biol., Mar 1999; 65: 281.

Product Categories/Family for anti-CCR5 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4502639
• NCBI GeneID: 1234
• NCBI Accession #: NP_000570.1
• NCBI GenBank Nucleotide #: NM_000579.3
• UniProt Accession #: P51681; O14692; O14693; O14695; O14696; O14697; O14698; O14699; O14700; O14701; O14702; O14703
• Molecular Weight: 40,524 Da
• NCBI Official Full Name: C-C chemokine receptor type 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 5 (gene/pseudogene)
• NCBI Official Symbol: CCR5
• NCBI Official Synonym Symbols: CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
• NCBI Protein Information: C-C chemokine receptor type 5
• UniProt Protein Name: C-C chemokine receptor type 5
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR5
• UniProt Synonym Gene Names: CMKBR5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5
• UniProt Entry Name: CCR5_HUMAN

NCBI Description

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2015]

Uniprot Description

CCR5: a 7-transmembrane G-linked receptor for a number of inflammatory C-C type chemokines including MIP-1-alpha, MIP-1-beta and RANTES. Transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (along with CD4) for HIV-1 R5 isolates. Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCR4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on S6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2. Variations in CCR5 are associated with resistance or susceptibility to immunodeficiency virus type 1 (resistance or susceptibility to HIV-1). Variations in CCR5 gene also influence the rate of progression to AIDS after infection. R60S variant, a naturally occurring mutation in a conserved residue in the first intracellular domain of CCR5, results in reduced amounts of the protein in the membrane and consequently may be associated with reduced susceptibility to infection by microbes that depend on these molecules as their receptors. Variations in CCR5 are associated with susceptibility to West Nile virus (WNV) infection

Protein type: Receptor, cytokine; Motility/polarity/chemotaxis; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity; coreceptor activity; C-C chemokine binding; actin binding; phosphoinositide phospholipase C activity

Biological Process: viral reproduction; calcium-mediated signaling; MAPKKK cascade; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; calcium ion transport; dendritic cell chemotaxis; cellular defense response; entry into host cell; immune response; inflammatory response

Disease: West Nile Virus, Susceptibility To; Diabetes Mellitus, Insulin-dependent, 22; Hepatitis C Virus, Susceptibility To