Rabbit anti-Human CCR4 Polyclonal Antibody | anti-CCR4 antibody

CCR4, CT (CCR4, CMKBR4, C-C chemokine receptor type 4, K5-5, CD194) (Azide free) (HRP)

Cat. Num. AAA6282612

• Gene Names: CCR4; CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCR4; Polyclonal Antibody; CT (CCR4; CMKBR4; C-C chemokine receptor type 4; K5-5; CD194) (Azide free) (HRP); CD194; anti-CCR4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CCR4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CCR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 amino acids from the C-terminal region of human CCR4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CCR4 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CCR4 antibody detected the CCR4 protein (arrow).)

Related Product Information for anti-CCR4 antibody

The protein encoded by this gene belongs to the G-protein-coupled receptor family. It is a receptor for the CC chemokine-MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

Product Categories/Family for anti-CCR4 antibody

Antibodies; CD Markers

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Han, S., et al. Hum. Immunol. 71(7):727-730(2010). Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010). Klapa, S., et al. Clin. Exp. Rheumatol. 28 (1 SUPPL 57), 72-80 (2010): Maneechotesuwan, K., et al. J Med Assoc Thai 93 SUPPL 1, S62-S70 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 5031627
• NCBI GeneID: 1233
• NCBI Accession #: NP_005499.1
• NCBI GenBank Nucleotide #: NM_005508.4
• UniProt Accession #: P51679; Q9ULY6; Q9ULY7
• Molecular Weight: 41,403 Da
• NCBI Official Full Name: C-C chemokine receptor type 4
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 4
• NCBI Official Symbol: CCR4
• NCBI Official Synonym Symbols: CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099
• NCBI Protein Information: C-C chemokine receptor type 4; CCR-4; C-C CKR-4; chemokine (C-C) receptor 4
• UniProt Protein Name: C-C chemokine receptor type 4
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR4
• UniProt Synonym Gene Names: CMKBR4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4
• UniProt Entry Name: CCR4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq, Jul 2008]

Uniprot Description

CCR4: High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol- calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p24

Cellular Component: cell soma; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: C-C chemokine receptor activity; chemokine receptor activity

Biological Process: response to antibiotic; G-protein coupled receptor protein signaling pathway; response to radiation; elevation of cytosolic calcium ion concentration; positive regulation of positive chemotaxis; response to bacterium; neuron migration; immune response; chemotaxis; inflammatory response; tolerance induction