Rabbit CCNT1 Polyclonal Antibody | anti-CCNT1 antibody

Rabbit Anti Human CCNT1 PolyClonal Antibody

Cat. Num. AAA1489553

• Gene Names: CCNT1; CCNT; CYCT1; HIVE1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: CCNT1; Polyclonal Antibody; Rabbit Anti Human CCNT1 PolyClonal Antibody; CCNT; CYCT1; HIVE1; Cyclin T1; anti-CCNT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 726

• Applicable Applications for anti-CCNT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: A synthetic peptide of human CCNT1
• Relevance: Positive transcription elongation factor (P-TEFb) is a heterodimer composed of cyclin T proteins and CDK9. P-TEFb plays a critical role in the transition of the RNA polymerase II (RNAPII) machinery from transcription initiation to elongation. At some genes during transcription initiation, RNAPII moves approximately 50 nucleotides away from the transcription start site into the gene where it then pauses and awaits signaling for the formation of a productive transcription elongation complex. The release of this promoter proximal pausing of RNAPII is signaled by phosphorylation of the C-terminal domain (CTD) within the largest subunit of RNAPII at Ser2 of the heptapeptide repeat sequence by P-TEFb. This phosphorylation event is important for the recruitment of mRNA processing factors and chromatin modifiers that are necessary for proper gene expression. P-TEFb also promotes transcription elongation by phosphorylating DSIF (DRB-induced stimulating factor) and NELF (negative elongation factor), two negative elongation factors that retain RNAPII at the promoter proximal region of genes to initiate transcription elongation.
• Storage Buffer: Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

NCBI and Uniprot Product Information

• NCBI GI #: 9296942
• NCBI GeneID: 904
• Molecular Weight: 21,206 Da
• NCBI Official Full Name: Cyclin-T1
• NCBI Official Synonym Full Names: cyclin T1
• NCBI Official Symbol: CCNT1
• NCBI Official Synonym Symbols: CCNT; CYCT1; HIVE1
• NCBI Protein Information: cyclin-T1; cyclin C-related protein; CDK9-associated C-type protein; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
• UniProt Protein Name: Cyclin-T1
• Protein Family: Cyclin
• UniProt Gene Name: CCNT1
• UniProt Synonym Gene Names: CycT1; Cyclin-T
• UniProt Entry Name: CCNT1_HUMAN

NCBI Description

This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

CCNT1: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Interacts with the transactivation region of HIV-1, HIV-2 and SIV Tat. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with MDFIC. Ubiquitously expressed. Belongs to the cyclin family. Cyclin C subfamily.

Protein type: Transcription initiation complex; Cell cycle regulation

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: nucleoplasm; transcription elongation factor complex b; nucleolus; nucleus

Molecular Function: protein binding; snRNA binding; DNA binding; chromatin binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; transcription, DNA-dependent; positive regulation of viral transcription; cell cycle; protein amino acid phosphorylation; cell division; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of cyclin-dependent protein kinase activity