Mouse JE/MCP-1 ELISA Kit | JE/MCP-1 elisa kit

JE/MCP-1 (Murine) OmniKine ELISA Kit

Cat. Num. AAA9501915

• Gene Names: Ccl2; JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594
• Reactivity: Mouse
• Synonyms: JE/MCP-1; JE/MCP-1 (Murine) OmniKine ELISA Kit; C-C motif chemokine 2; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; Platelet-derived growth factor-inducible protein JE; Small-inducible cytokine A2; Scya2; JE/MCP-1 elisa kit

• Reactivity: Mouse
• Specificity: The OmniKine Murine JE/MCP-1 ELISA is capable of recognizing both recombinant and naturally produced Murine JE/MCP-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.; Rat: MCP-1; The antigens listed below were tested at 50 ng/ml and exhibited less than 2% cross reactivity.; Murine: MCP-3; The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.; Human: MCP-1, MCP-3; Murine: MCP-5; The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.; Human: CTACK, MCP-2, MIP-4, MIP-1beta; Murine: C10, CTACK, Eotaxin, KC, MCP-2, MDC, MIP-1alpha, MIP-1beta, MIP-1gamma, MIP-3beta, RANTES
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 148

• Samples: Detection And Quantification Of Human Pcsk9 Concentrations In Cell Lysates, Sera And Plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: 125-8000 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for JE/MCP-1 elisa kit

Principle of the Assay: The OmniKine Human PCSK9 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human PCSK9 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human PCSK9 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Proprotein Convertase 9/PCSK9 may be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. The enzyme is inhibited by EGTA. PCSK9 is expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. Variant Leu-23 insertion polymorphism in PCSK9 might have a modifier effect on LDLR mutation and familial hypercholesterolemia. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3), a familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins.

NCBI and Uniprot Product Information

• NCBI GI #: 6755430
• NCBI GeneID: 20296
• NCBI Accession #: NP_035463.1
• NCBI GenBank Nucleotide #: NM_011333.3
• UniProt Accession #: P10148; Q9QYD7
• Molecular Weight: 16,326 Da
• NCBI Official Full Name: C-C motif chemokine 2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 2
• NCBI Official Symbol: Ccl2
• NCBI Official Synonym Symbols: JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594
• NCBI Protein Information: C-C motif chemokine 2
• UniProt Protein Name: C-C motif chemokine 2
• UniProt Gene Name: Ccl2
• UniProt Synonym Gene Names: Je; Mcp1; Scya2; MCP-1

NCBI Description

This gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. [provided by RefSeq, Sep 2015]

Uniprot Description

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11 C|11 49.82 cM

Cellular Component: axon terminus; cell soma; cytoplasm; dendrite; endocytic vesicle; extracellular region; extracellular space; perikaryon; perinuclear region of cytoplasm; rough endoplasmic reticulum; synapse

Molecular Function: CCR2 chemokine receptor binding; chemokine activity; cytokine activity; G-protein coupled receptor binding; heparin binding; protein binding

Biological Process: angiogenesis; cellular calcium ion homeostasis; chemotaxis; cytokine-mediated signaling pathway; cytoskeleton organization; eosinophil chemotaxis; G-protein coupled receptor signaling pathway; glial cell migration; immune response; inflammatory response; killing of cells of other organism; leukocyte migration involved in inflammatory response; lymphocyte chemotaxis; macrophage chemotaxis; negative regulation of angiogenesis; neutrophil chemotaxis; positive regulation of cell adhesion; positive regulation of cell-cell adhesion; positive regulation of cellular extravasation; positive regulation of collagen biosynthetic process; positive regulation of endothelial cell proliferation; positive regulation of GTPase activity; positive regulation of leukocyte mediated cytotoxicity; positive regulation of leukocyte migration; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of synaptic transmission; positive regulation of T cell activation; positive regulation of tumor necrosis factor production; regulation of cell shape; response to heat; response to wounding; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway