Rabbit anti-Human CCNE1 Polyclonal Antibody | anti-CCNE1 antibody

CCNE1, CT (G1/S-specific Cyclin-E1, CCNE) (APC)

Cat. Num. AAA6471241

• Gene Names: CCNE1; CCNE
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: CCNE1; Polyclonal Antibody; CT (G1/S-specific Cyclin-E1; CCNE) (APC); anti-CCNE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Cyclin E1 at ~47kD.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CCNE1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:50-1:100; IHC-P 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to the C-terminal region of human Cyclin E1 conjugated to KLH (P24864).
• Conjugate: APC
• Positive Control: A2058 cell line lysates, human testis tissue and NCI-H292 cells.
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Cyclin E1 (C-term) Pab in A2058 cell line lysates (35ug/lane). Cyclin E1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue with Cyclin E1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using Cyclin E1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CCNE1 antibody

Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E1 forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E1 accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of Cyclin E1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Product Categories/Family for anti-CCNE1 antibody

Antibodies; Cyclin

References

1. Ausserlechner, M.J., et al., Leukemia 19(6):1051-1057 (2005). 2. Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005). 3. Honda, R., et al., EMBO J. 24(3):452-463 (2005). 4. Brzezinski, J., et al., Clin. Cancer Res. 11(3):1037-1043 (2005). 5. Hayami, R., et al., Cancer Res. 65(1):6-10 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 17318559
• NCBI GeneID: 898
• NCBI Accession #: NP_001229
• NCBI GenBank Nucleotide #: NM_001238
• UniProt Accession #: P24864; Q14091; Q8NFG1; Q92501; Q9UD21; A8K684
• Molecular Weight: 45,150 Da
• NCBI Official Full Name: G1/S-specific cyclin-E1
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: CCNE1
• NCBI Official Synonym Symbols: CCNE
• NCBI Protein Information: G1/S-specific cyclin-E1; cyclin E variant ex5del; cyclin E variant ex7del; cyclin Es; cyclin Et
• UniProt Protein Name: G1/S-specific cyclin-E1
• UniProt Gene Name: CCNE1
• UniProt Synonym Gene Names: CCNE
• UniProt Entry Name: CCNE1_HUMAN

Uniprot Description

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: protein binding; androgen receptor binding; transcription coactivator activity; kinase activity; cyclin-dependent protein kinase regulator activity; protein kinase binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; Wnt receptor signaling pathway; DNA replication initiation; cell division; regulation of cell cycle; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; mitotic cell cycle; regulation of protein kinase activity; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle